9L7D
Type III-B CRISPR-Cas effector from Archaeoglobus fulgidus (Form 2)
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, G (F, E) | Type III-B CRISPR module RAMP protein Cmr4 | polymer | 369 | 41252.3 | 2 | UniProt (O28416) Pfam (PF03787) | Archaeoglobus fulgidus DSM 4304 | |
| 2 | B (A) | Cmr1 C-terminal domain-containing protein | polymer | 329 | 38017.0 | 1 | UniProt (O28411) Pfam (PF03787) Pfam (PF22407) | Archaeoglobus fulgidus | |
| 3 | C (B) | GGDEF domain-containing protein | polymer | 1004 | 115211.9 | 1 | UniProt (O28412) Pfam (PF12469) Pfam (PF22335) | Archaeoglobus fulgidus DSM 4304 | |
| 4 | D (C) | Type III-B CRISPR module-associated protein Cmr3 | polymer | 341 | 39043.3 | 1 | UniProt (O28413) Pfam (PF09700) | Archaeoglobus fulgidus DSM 4304 | |
| 5 | E (I) | Type III-B CRISPR module RAMP protein Cmr6 | polymer | 343 | 39469.9 | 1 | UniProt (O28418) Pfam (PF03787) | Archaeoglobus fulgidus DSM 4304 | |
| 6 | F (G) | CRISPR system Cmr subunit Cmr5 | polymer | 155 | 17407.7 | 1 | UniProt (O28417) Pfam (PF09701) | Archaeoglobus fulgidus DSM 4304 | CRISPR type III-B/RAMP module-associated protein Cmr5 |
| 7 | H (V) | RNA (5'-R(*UP*GP*UP*AP*GP*UP*AP*UP*GP*CP*GP*GP*UP*CP*CP*UP*UP*GP*CP*GP*GP*C)-3') | polymer | 45 | 14501.6 | 1 | Pyrococcus furiosus DSM 3638 | ||
| 8 | I (B) | MAGNESIUM ION | non-polymer | 24.3 | 1 | Chemie (MG) | |||
| 9 | J, K (B) | ADENOSINE-5'-TRIPHOSPHATE | non-polymer | 507.2 | 2 | Chemie (ATP) | |||
| 10 | L (B) | ZINC ION | non-polymer | 65.4 | 1 | Chemie (ZN) |
Sequence modifications
F, E: 1 - 355 (UniProt: O28416)
| PDB | External Database | Details |
|---|---|---|
| Met -13 | - | initiating methionine |
| Gly -12 | - | expression tag |
| Ser -11 | - | expression tag |
| Ser -10 | - | expression tag |
| His -9 | - | expression tag |
| His -8 | - | expression tag |
| His -7 | - | expression tag |
| His -6 | - | expression tag |
| His -5 | - | expression tag |
| His -4 | - | expression tag |
| Ser -3 | - | expression tag |
| Gln -2 | - | expression tag |
| Asp -1 | - | expression tag |
| Pro 0 | - | expression tag |
| Ala 31 | Asp 31 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 8 |
| Total formula weight | 346155.9 | |
| Non-Polymers* | Number of molecules | 4 |
| Total formula weight | 1104.1 | |
| All* | Total formula weight | 347259.9 |
