9L6Y

Crystal structure of the L7Ae derivative protein LS12 in complex with its co-evolved target CS2 RNA

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C (A, C)	Large ribosomal subunit protein eL8	polymer	119	13100.1	2	UniProt (O29494) Pfam (PF01248)	Archaeoglobus fulgidus (strain ATCC 49558 / DSM 4304 / JCM 9628 / NBRC 100126 / VC-16)	50S ribosomal protein L7Ae,Ribosomal protein L8e
2	B, D (B, D)	CS2 RNA	polymer	33	10655.4	2		synthetic construct
3	E, F, G, H, I (B, D)	CALCIUM ION	non-polymer		40.1	5	Chemie (CA)

Sequence modifications

A, C: 2 - 119 (UniProt: O29494)

PDB	External Database	Details
Ser 1	-	expression tag
Ser 34	Glu 34	engineered mutation
Leu 37	Lys 37	engineered mutation
Ser 40	Glu 40	engineered mutation
Arg 88	Ile 88	engineered mutation
Val 89	Glu 89	engineered mutation
Tyr 90	Val 90	engineered mutation
Gly 92	Cys 92	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	47511.1
Non-Polymers*	Number of molecules	5
	Total formula weight	200.4
All*	Total formula weight	47711.5

*Water molecules are not included.