9L6F

Crystal structure of KRas G12D (GDP) in complex with ASP3082

This is a non-PDB format compatible entry.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, E, I, M (A, E, I, M)	von Hippel-Lindau disease tumor suppressor	polymer	163	18840.4	4	UniProt (P40337) Pfam (PF01847) Pfam (PF17211)	Homo sapiens (human)	Protein G7,pVHL
2	B, F, J, N (B, F, J, N)	Elongin-C	polymer	96	10843.4	4	UniProt (Q15369) Pfam (PF03931)	Homo sapiens (human)	EloC,Elongin 15 kDa subunit,RNA polymerase II transcription factor SIII subunit C,SIII p15,Transcription elongation factor B polypeptide 1
3	C, G, K, O (C, G, K, O)	Elongin-B	polymer	118	13147.8	4	UniProt (Q15370) Pfam (PF00240)	Homo sapiens (human)	EloB,Elongin 18 kDa subunit,RNA polymerase II transcription factor SIII subunit B,SIII p18,Transcription elongation factor B polypeptide 2
4	D, H, L, P (D, H, L, P)	Isoform 2B of GTPase KRas	polymer	170	19386.8	4	UniProt (P01116) Pfam (PF00071)	Homo sapiens (human)	K-Ras 2,Ki-Ras,c-K-ras,c-Ki-ras
5	BA, Q, V, Y (P, A, H, L)	ASP3082	non-polymer		1117.3	4	Chemie (A1L66)
6	R, T, W, Z (D, H, L, P)	GUANOSINE-5'-DIPHOSPHATE	non-polymer		443.2	4	Chemie (GDP)
7	AA, S, U, X (P, D, H, L)	MAGNESIUM ION	non-polymer		24.3	4	Chemie (MG)

Sequence modifications

A, E, I, M: 54 - 213 (UniProt: P40337)

PDB	External Database	Details
Gly 51	-	expression tag
Ser 52	-	expression tag
His 53	-	expression tag

D, H, L, P: 1 - 169 (UniProt: P01116)

PDB	External Database	Details
Gly 0	-	expression tag
Asp 12	Gly 12	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	16
	Total formula weight	248873.9
Non-Polymers*	Number of molecules	12
	Total formula weight	6339.2
All*	Total formula weight	255213.2

*Water molecules are not included.