9L5O
Crystal structure of the mutant Y549A of collagenase VhaC
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | microbial collagenase | polymer | 532 | 59802.5 | 1 | UniProt (A0A8B3DGT3) | Vibrio harveyi VHJR7 | |
| 2 | B (B) | LEU-SER-GLU-PRO-SER-GLN-GLN-VAL-THR-GLU-ILE-TYR-GLN-HIS-HIS-ALA-HIS-GLN-ASN-GLY-ASN | polymer | 21 | 2420.5 | 1 | Vibrio harveyi VHJR7 | ||
| 3 | C (C) | ASP-TYR-ALA-PRO-THR-LYS-LEU-LEU-PRO-GLN-GLN-PRO | polymer | 12 | 1371.6 | 1 | Vibrio harveyi VHJR7 | ||
| 4 | D, E, F, G, H... (A) | GLYCEROL | non-polymer | 92.1 | 8 | Chemie (GOL) PubChem (753) | |||
| 5 | L, M, N (A) | CALCIUM ION | non-polymer | 40.1 | 3 | Chemie (CA) PubChem (271) | |||
| 6 | O (A) | ZINC ION | non-polymer | 65.4 | 1 | Chemie (ZN) | |||
| 7 | P, Q, R (A, B, C) | water | water | 18.0 | 494 | Chemie (HOH) |
Sequence modifications
A: 78 - 605 (UniProt: A0A8B3DGT3)
| PDB | External Database | Details |
|---|---|---|
| Asp 132 | Asn 132 | conflict |
| Ala 267 | Val 267 | conflict |
| Ala 549 | Tyr 549 | engineered mutation |
| Leu 606 | - | expression tag |
| Glu 607 | - | expression tag |
| His 608 | - | expression tag |
| His 609 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 3 |
| Total formula weight | 63594.6 | |
| Non-Polymers* | Number of molecules | 12 |
| Total formula weight | 922.4 | |
| All* | Total formula weight | 64517.0 |
