9L42

Cryo-EM structure of human histamine receptor H4R in complex with agonist histamine and Gi proteins

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Histamine receptor H4R	polymer	312	36049.3	1	Pfam (PF00001) UniProt (by SIFTS) (Q9H3N8)	Homo sapiens (human)
2	B (B)	Guanine nucleotide-binding protein G(i) subunit alpha-1	polymer	354	40414.0	1	UniProt (P63096) Pfam (PF00503)	Homo sapiens (human)	Adenylate cyclase-inhibiting G alpha protein
3	C (C)	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	354	39086.6	1	UniProt (P62873) Pfam (PF25391)	Homo sapiens (human)	Transducin beta chain 1
4	D (D)	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	polymer	81	9242.6	1	UniProt (P59768) Pfam (PF00631)	Homo sapiens (human)	G gamma-I
5	E (E)	Antibody fragment scFv16	polymer	292	31347.1	1		synthetic construct (human)
6	F (A)	CHOLESTEROL	non-polymer		386.7	1	Chemie (CLR)
7	G (A)	HISTAMINE	non-polymer		111.1	1	Chemie (HSM)

Sequence modifications

B: 1 - 354 (UniProt: P63096)

PDB	External Database	Details
Asn 47	Ser 47	engineered mutation
Ala 203	Gly 203	engineered mutation
Ala 245	Glu 245	engineered mutation
Ser 326	Ala 326	engineered mutation

C: 2 - 340 (UniProt: P62873)

PDB	External Database	Details
Met -13	-	initiating methionine
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
His -9	-	expression tag
His -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
Gly -2	-	linker
Ser -1	-	linker
Ser 0	-	linker
Gly 1	-	linker

D: 2 - 71 (UniProt: P59768)

PDB	External Database	Details
Met -9	-	initiating methionine
His -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
His -1	-	expression tag
His 0	-	expression tag
His 1	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	156139.7
Non-Polymers*	Number of molecules	2
	Total formula weight	497.8
All*	Total formula weight	156637.5

*Water molecules are not included.