9L3Y

Cryo-EM structure of the G-protein coupled receptor 1 (GPR1) in complex with chemerin and Gi1

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (R)	Chemerin-like receptor 2	polymer	368	42771.6	1	UniProt (P46091) Pfam (PF00001)	Homo sapiens (human)	G-protein coupled receptor 1 (GPR1),Chemerin chemokine-like receptor 2,Chemokine-like receptor 2
2	B (L)	Retinoic acid receptor responder protein 2	polymer	137	15904.2	1	UniProt (Q99969)	Homo sapiens (human)	Chemerin,RAR-responsive protein TIG2,Tazarotene-induced gene 2 protein
3	C (A)	Guanine nucleotide-binding protein G(i) subunit alpha-1	polymer	354	40447.1	1	UniProt (P63096) Pfam (PF00503)	Homo sapiens (human)	Adenylate cyclase-inhibiting G alpha protein
4	D (B)	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	340	37416.9	1	UniProt (P62873) Pfam (PF25391)	Homo sapiens (human)	Transducin beta chain 1
5	E (C)	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	polymer	71	7861.1	1	UniProt (P59768) Pfam (PF00631)	Homo sapiens (human)	G gamma-I

Sequence modifications

R: 1 - 322 (UniProt: P46091)

PDB	External Database	Details
Glu 323	-	expression tag
Phe 324	-	expression tag
Leu 325	-	expression tag
Glu 326	-	expression tag
Val 327	-	expression tag
Leu 328	-	expression tag
Phe 329	-	expression tag
Gln 330	-	expression tag
Gly 331	-	expression tag
Pro 332	-	expression tag
Trp 333	-	expression tag
Ser 334	-	expression tag
His 335	-	expression tag
Pro 336	-	expression tag
Gln 337	-	expression tag
Phe 338	-	expression tag
Glu 339	-	expression tag
Lys 340	-	expression tag
Gly 341	-	expression tag
Gly 342	-	expression tag
Gly 343	-	expression tag
Ser 344	-	expression tag
Gly 345	-	expression tag
Gly 346	-	expression tag
Gly 347	-	expression tag
Ser 348	-	expression tag
Gly 349	-	expression tag
Gly 350	-	expression tag
Ser 351	-	expression tag
Ala 352	-	expression tag
Trp 353	-	expression tag
Ser 354	-	expression tag
His 355	-	expression tag
Pro 356	-	expression tag
Gln 357	-	expression tag
Phe 358	-	expression tag
Glu 359	-	expression tag
Lys 360	-	expression tag
Asp 361	-	expression tag
Tyr 362	-	expression tag
Lys 363	-	expression tag
Asp 364	-	expression tag
Asp 365	-	expression tag
Asp 366	-	expression tag
Asp 367	-	expression tag
Lys 368	-	expression tag

A: 1 - 354 (UniProt: P63096)

PDB	External Database	Details
Cys 47	Ser 47	engineered mutation
Thr 202	Gly 202	engineered mutation
Ala 203	Gly 203	engineered mutation
Ala 245	Glu 245	engineered mutation
Ser 326	Ala 326	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	144401.0
All*	Total formula weight	144401.0

*Water molecules are not included.