9L1P

Cryo-EM structure of the ICT01-BTN2A1/BTN3A1/BTN3A2 complex, local refinement

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (B)	Butyrophilin subfamily 2 member A1	polymer	534	60684.5	1	UniProt (Q7KYR7) Pfam (PF07686) Pfam (PF22705) Pfam (PF13765) Pfam (PF00622)	Homo sapiens (human)
2	B (E)	Butyrophilin subfamily 3 member A2	polymer	340	37175.1	1	UniProt (P78410) Pfam (PF07686) Pfam (PF22705)	Homo sapiens (human)
3	C (H)	ICT01 heavy chain	polymer	255	27789.4	1		Homo sapiens
4	D (L)	ICT01 light chain	polymer	236	25968.2	1		Homo sapiens

Sequence modifications

B: 29 - 527 (UniProt: Q7KYR7)

PDB	External Database	Details
Met 7	-	initiating methionine
Asp 8	-	expression tag
Met 9	-	expression tag
Arg 10	-	expression tag
Val 11	-	expression tag
Pro 12	-	expression tag
Ala 13	-	expression tag
Gln 14	-	expression tag
Leu 15	-	expression tag
Leu 16	-	expression tag
Gly 17	-	expression tag
Leu 18	-	expression tag
Leu 19	-	expression tag
Leu 20	-	expression tag
Leu 21	-	expression tag
Trp 22	-	expression tag
Leu 23	-	expression tag
Ser 24	-	expression tag
Gly 25	-	expression tag
Ala 26	-	expression tag
Arg 27	-	expression tag
Cys 28	-	expression tag
Gly 528	-	expression tag
Ser 529	-	expression tag
Ser 530	-	expression tag
Gly 531	-	expression tag
Met 532	-	expression tag
Asp 533	-	expression tag
Tyr 534	-	expression tag
Lys 535	-	expression tag
Asp 536	-	expression tag
Asp 537	-	expression tag
Asp 538	-	expression tag
Asp 539	-	expression tag
Lys 540	-	expression tag

E: 30 - 334 (UniProt: P78410)

PDB	External Database	Details
Met 8	-	initiating methionine
Asp 9	-	expression tag
Met 10	-	expression tag
Arg 11	-	expression tag
Val 12	-	expression tag
Pro 13	-	expression tag
Ala 14	-	expression tag
Gln 15	-	expression tag
Leu 16	-	expression tag
Leu 17	-	expression tag
Gly 18	-	expression tag
Leu 19	-	expression tag
Leu 20	-	expression tag
Leu 21	-	expression tag
Leu 22	-	expression tag
Trp 23	-	expression tag
Leu 24	-	expression tag
Ser 25	-	expression tag
Gly 26	-	expression tag
Ala 27	-	expression tag
Arg 28	-	expression tag
Cys 29	-	expression tag
Gly 335	-	expression tag
Ser 336	-	expression tag
Ser 337	-	expression tag
Gly 338	-	expression tag
Met 339	-	expression tag
Asp 340	-	expression tag
Tyr 341	-	expression tag
Lys 342	-	expression tag
Asp 343	-	expression tag
Asp 344	-	expression tag
Asp 345	-	expression tag
Asp 346	-	expression tag
Lys 347	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	151617.1
All*	Total formula weight	151617.1

*Water molecules are not included.