9KW6

Polyether epoxide hydrolase MonBI-MonBII complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, D (A, B, D)	MonBI,MonBII	polymer	302	33356.7	3	UniProt (Q846W7) UniProt (Q846W8)	Streptomyces virginiae	Probable monensin biosynthesis isomerase
2	C (C)	MonBI,MonBII	polymer	302	33382.7	1	UniProt (Q846W7) UniProt (Q846W8)	Streptomyces virginiae	Probable monensin biosynthesis isomerase
3	E, G, H, I, L... (A, B, C, D)	GLYCEROL	non-polymer		92.1	7	Chemie (GOL)
4	F, J, K (B, D)	DI(HYDROXYETHYL)ETHER	non-polymer		106.1	3	Chemie (PEG)
5	O, P, Q, R (A, B, C, D)	water	water		18.0	145	Chemie (HOH)

Sequence modifications

A, B, D: 2 - 141 (UniProt: Q846W7)

PDB	External Database	Details
Met -14	-	initiating methionine
Asn -13	-	expression tag
His -12	-	expression tag
Lys -11	-	expression tag
Val -10	-	expression tag
His -9	-	expression tag
His -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
Ile -3	-	expression tag
Glu -2	-	expression tag
Gly -1	-	expression tag
Ala 0	-	expression tag
Ala 1	-	expression tag
Ser 142	-	linker
Gly 143	-	linker
Gly 144	-	linker
Ser 160	-	linker
Gly 161	-	linker
Pro 162	-	linker
Ala 163	-	linker
Ala 164	-	linker

A, B, D: 165 - 287 (UniProt: Q846W8)

PDB	External Database	Details
Arg 288	-	expression tag
Val 289	-	expression tag
Gln 290	-	expression tag
Ala 291	-	expression tag
Phe 292	-	expression tag
Trp 293	-	expression tag
Gly 294	-	expression tag
Val 295	-	expression tag
Thr 296	-	expression tag
Asp 297	-	expression tag
Val 298	-	expression tag
Thr 299	-	expression tag
Met 300	-	expression tag
Asp 301	-	expression tag
Asp 302	-	expression tag

C: 2 - 141 (UniProt: Q846W7)

PDB	External Database	Details
Met -14	-	initiating methionine
Asn -13	-	expression tag
His -12	-	expression tag
Lys -11	-	expression tag
Val -10	-	expression tag
His -9	-	expression tag
His -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
Ile -3	-	expression tag
Glu -2	-	expression tag
Gly -1	-	expression tag
Ala 0	-	expression tag
Ala 1	-	expression tag
Ser 142	-	linker
Gly 143	-	linker
Gly 144	-	linker
Ser 160	-	linker
Gly 161	-	linker
Pro 162	-	linker
Ala 163	-	linker

C: 164 - 287 (UniProt: Q846W8)

PDB	External Database	Details
Arg 288	-	expression tag
Val 289	-	expression tag
Gln 290	-	expression tag
Ala 291	-	expression tag
Phe 292	-	expression tag
Trp 293	-	expression tag
Gly 294	-	expression tag
Val 295	-	expression tag
Thr 296	-	expression tag
Asp 297	-	expression tag
Val 298	-	expression tag
Thr 299	-	expression tag
Met 300	-	expression tag
Asp 301	-	expression tag
Asp 302	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	133452.9
Non-Polymers*	Number of molecules	10
	Total formula weight	963.0
All*	Total formula weight	134415.9

*Water molecules are not included.