9KTD

Fe(II)-bound CpfC (HemH) Y13C variant modified with bromobimane

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Coproporphyrin III ferrochelatase	polymer	326	37169.7	1	UniProt (P32396) Pfam (PF00762)	Bacillus subtilis subsp. subtilis str. 168	Water-soluble ferrochelatase
2	B, C (A)	MAGNESIUM ION	non-polymer		24.3	2	Chemie (MG)
3	D, E, F, G, H (A)	CHLORIDE ION	non-polymer		35.5	5	Chemie (CL)
4	I (A)	3-(bromomethyl)-2,5,6-trimethyl-1H,7H-pyrazolo[1,2-a]pyrazole-1,7-dione	non-polymer		271.1	1	Chemie (9UM)
5	J, K, L, M, N (A)	FE (II) ION	non-polymer		55.8	5	Chemie (FE2)
6	O (A)	water	water		18.0	354	Chemie (HOH)

Sequence modifications

A: 0 - 310 (UniProt: P32396)

PDB	External Database	Details
Val 1	-	insertion
Cys 13	Tyr 13	engineered mutation
Val 311	-	expression tag
Asp 312	-	expression tag
Lys 313	-	expression tag
Leu 314	-	expression tag
Ala 315	-	expression tag
Ala 316	-	expression tag
Ala 317	-	expression tag
Leu 318	-	expression tag
Glu 319	-	expression tag
His 320	-	expression tag
His 321	-	expression tag
His 322	-	expression tag
His 323	-	expression tag
His 324	-	expression tag
His 325	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	37169.7
Non-Polymers*	Number of molecules	13
	Total formula weight	776.2
All*	Total formula weight	37945.9

*Water molecules are not included.