9KTB

Bacillus subtilis CpfC (HemH) Y13C variant modified with bromobimane

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Coproporphyrin III ferrochelatase	polymer	326	37169.7	1	UniProt (P32396) Pfam (PF00762)	Bacillus subtilis subsp. subtilis str. 168	Water-soluble ferrochelatase
2	B (A)	3-(bromomethyl)-2,5,6-trimethyl-1H,7H-pyrazolo[1,2-a]pyrazole-1,7-dione	non-polymer		271.1	1	Chemie (9UM)
3	C, D, E, F, G (A)	MAGNESIUM ION	non-polymer		24.3	5	Chemie (MG)
4	H (A)	CHLORIDE ION	non-polymer		35.5	1	Chemie (CL)
5	I (A)	water	water		18.0	467	Chemie (HOH)

Sequence modifications

A: 0 - 310 (UniProt: P32396)

PDB	External Database	Details
Val 1	-	insertion
Cys 13	Tyr 13	engineered mutation
Val 311	-	expression tag
Asp 312	-	expression tag
Lys 313	-	expression tag
Leu 314	-	expression tag
Ala 315	-	expression tag
Ala 316	-	expression tag
Ala 317	-	expression tag
Leu 318	-	expression tag
Glu 319	-	expression tag
His 320	-	expression tag
His 321	-	expression tag
His 322	-	expression tag
His 323	-	expression tag
His 324	-	expression tag
His 325	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	37169.7
Non-Polymers*	Number of molecules	7
	Total formula weight	428.1
All*	Total formula weight	37597.8

*Water molecules are not included.