9KQO
cryo-EM structure of RNF20/RNF40-RAD6A-Ub in complex with H2BS112GlcNAc nucleosome
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | E3 ubiquitin-protein ligase BRE1B | polymer | 60 | 7120.4 | 1 | UniProt (O75150) Pfam (PF00097) | Homo sapiens (human) | BRE1-B,95 kDa retinoblastoma-associated protein,RBP95,RING finger protein 40,RING-type E3 ubiquitin transferase BRE1B |
| 2 | B (B) | Polyubiquitin-B | polymer | 75 | 8519.8 | 1 | UniProt (J3QS39) Pfam (PF00240) | Homo sapiens (human) | |
| 3 | C, G (C, G) | Histone H2A type 1-B/E | polymer | 129 | 14034.4 | 2 | UniProt (P04908) Pfam (PF00125) Pfam (PF16211) | Homo sapiens (human) | Histone H2A.2,Histone H2A/a,Histone H2A/m |
| 4 | D, H (D, H) | Histone H2B type 1-K | polymer | 125 | 13764.0 | 2 | UniProt (O60814) Pfam (PF00125) | Homo sapiens (human) | H2B K,HIRA-interacting protein 1 |
| 5 | E, K (E, K) | Histone H3 | polymer | 135 | 15273.8 | 2 | UniProt (A0A653DHJ5) Pfam (PF00125) | Homo sapiens (human) | |
| 6 | F, L (F, L) | Histone H4 | polymer | 102 | 11263.2 | 2 | UniProt (P62805) Pfam (PF15511) | Homo sapiens (human) | |
| 7 | I (I) | DNA (147-MER) | polymer | 147 | 45604.0 | 1 | Homo sapiens | ||
| 8 | J (J) | DNA (147-MER) | polymer | 147 | 45145.8 | 1 | Homo sapiens | ||
| 9 | M (M) | E3 ubiquitin-protein ligase BRE1A | polymer | 60 | 7144.5 | 1 | UniProt (Q5VTR2) Pfam (PF00097) | Homo sapiens (human) | BRE1-A,hBRE1,RING finger protein 20,RING-type E3 ubiquitin transferase BRE1A |
| 10 | N (R) | Ubiquitin-conjugating enzyme E2 A | polymer | 150 | 17114.1 | 1 | UniProt (P49459) Pfam (PF00179) | Homo sapiens (human) | E2 ubiquitin-conjugating enzyme A,RAD6 homolog A,HR6A,hHR6A,Ubiquitin carrier protein A,Ubiquitin-protein ligase A |
| 11 | O, P, R, S (A, M) | ZINC ION | non-polymer | 65.4 | 4 | Chemie (ZN) | |||
| 12 | Q (D) | 2-acetamido-2-deoxy-beta-D-glucopyranose | non-polymer | 221.2 | 1 | Chemie (NAG) |
Sequence modifications
D, H: 1 - 125 (UniProt: O60814)
| PDB | External Database | Details |
|---|---|---|
| Cys 120 | Lys 121 | conflict |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 14 |
| Total formula weight | 239319.4 | |
| Non-Polymers* | Number of molecules | 5 |
| Total formula weight | 482.8 | |
| All* | Total formula weight | 239802.2 |
