9KQM

Cryo-EM structure of human VAChT in complex with VSM

This is a non-PDB format compatible entry.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Soluble cytochrome b562,Vesicular acetylcholine transporter	polymer	637	69325.8	1	UniProt (P0ABE7) UniProt (Q16572)	Escherichia coli	Cytochrome b-562,VAChT,Solute carrier family 18 member 3
2	B (A)	(1R,2R)-2-(4-phenylpiperidin-1-yl)cyclohexan-1-ol	non-polymer		259.4	1	Chemie (A1ER3)

Sequence modifications

A: -81 - 24 (UniProt: P0ABE7)

PDB	External Database	Details
Met -82	-	initiating methionine
Trp -75	Met 29	engineered mutation
Ile 20	His 124	engineered mutation
Leu 24	Arg 128	engineered mutation

A: 25 - 532 (UniProt: Q16572)

PDB	External Database	Details
His 533	-	expression tag
His 534	-	expression tag
His 535	-	expression tag
His 536	-	expression tag
His 537	-	expression tag
His 538	-	expression tag
His 539	-	expression tag
His 540	-	expression tag
His 541	-	expression tag
His 542	-	expression tag
Gly 543	-	expression tag
Ser 544	-	expression tag
Val 545	-	expression tag
Glu 546	-	expression tag
Asp 547	-	expression tag
Tyr 548	-	expression tag
Lys 549	-	expression tag
Asp 550	-	expression tag
Asp 551	-	expression tag
Asp 552	-	expression tag
Asp 553	-	expression tag
Lys 554	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	69325.8
Non-Polymers*	Number of molecules	1
	Total formula weight	259.4
All*	Total formula weight	69585.2

*Water molecules are not included.