9KPF

Cryo-EM structure of GPCR16-Gi complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Guanine nucleotide-binding protein G(i) subunit alpha-1	polymer	369	42293.9	1	UniProt (P63096) Pfam (PF00503)	Homo sapiens (human)	Adenylate cyclase-inhibiting G alpha protein
2	B (B)	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	366	39728.4	1	UniProt (P62873) Pfam (PF00400)	Homo sapiens (human)	Transducin beta chain 1
3	C (G)	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	polymer	71	7861.1	1	UniProt (P59768) Pfam (PF00631)	Homo sapiens (human)	G gamma-I
4	D (R)	Fusion protein 1,exo-alpha-sialidase,Taste receptor type 2 member 16,Fusion protein 2	polymer	1011	113855.8	1	UniProt (A0A4J1WX37) UniProt (Q9NYV7) Pfam (PF05296)	Homo sapiens	T2R16
5	E (S)	scFv16	polymer	266	28666.8	1		Homo sapiens
6	F (R)	2-(hydroxymethyl)phenyl beta-D-glucopyranoside	non-polymer		286.3	1	Chemie (SA0)

Sequence modifications

A: 1 - 354 (UniProt: P63096)

PDB	External Database	Details
Asp -14	-	expression tag
Tyr -13	-	expression tag
Lys -12	-	expression tag
Asp -11	-	expression tag
Asp -10	-	expression tag
Asp -9	-	expression tag
Asp -8	-	expression tag
Lys -7	-	expression tag
Glu -6	-	expression tag
Asn -5	-	expression tag
Leu -4	-	expression tag
Tyr -3	-	expression tag
Phe -2	-	expression tag
Gln -1	-	expression tag
Ser 0	-	expression tag

B: 1 - 340 (UniProt: P62873)

PDB	External Database	Details
Gly 341	-	expression tag
Ser 342	-	expression tag
Ser 343	-	expression tag
Gly 344	-	expression tag
Gly 345	-	expression tag
Gly 346	-	expression tag
Gly 347	-	expression tag
Ser 348	-	expression tag
Gly 349	-	expression tag
Gly 350	-	expression tag
Gly 351	-	expression tag
Gly 352	-	expression tag
Ser 353	-	expression tag
Ser 354	-	expression tag
Gly 355	-	expression tag
Val 356	-	expression tag
Ser 357	-	expression tag
Gly 358	-	expression tag
Trp 359	-	expression tag
Arg 360	-	expression tag
Leu 361	-	expression tag
Phe 362	-	expression tag
Lys 363	-	expression tag
Lys 364	-	expression tag
Ile 365	-	expression tag
Ser 366	-	expression tag

R: -537 - -479 (PDB: 9KPF)
R: -478 - -4 (UniProt: A0A4J1WX37)

PDB	External Database	Details
Gly -3	-	linker
Ser -2	-	linker
Gly -1	-	linker
Ser 0	-	linker
Gly 1	-	linker

R: 2 - 291 (UniProt: Q9NYV7)

PDB	External Database	Details
Cys 133	Ser 133	conflict

R: 292 - 473 (PDB: 9KPF)

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	232406.1
Non-Polymers*	Number of molecules	1
	Total formula weight	286.3
All*	Total formula weight	232692.4

*Water molecules are not included.