9KPD

Cryo-EM structure of GPCR16-miniGs complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Guanine nucleotide-binding protein G(s) subunit alpha isoforms short,Guanine nucleotide-binding protein G(s) subunit alpha isoforms short,Guanine nucleotide-binding protein G(t) subunit alpha-3	polymer	264	30583.3	1	UniProt (P63092) Pfam (PF00503)	Homo sapiens (human)	Adenylate cyclase-stimulating G alpha protein
2	B (B)	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	366	39728.4	1	UniProt (P62873) Pfam (PF25391)	Homo sapiens (human)	Transducin beta chain 1
3	C (G)	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	polymer	71	7861.1	1	UniProt (P59768) Pfam (PF00631)	Homo sapiens (human)	G gamma-I
4	D (N)	Nanobody 35	polymer	135	14845.5	1		Homo sapiens (human)
5	E (R)	Fusion protein 1,exo-alpha-sialidase,Taste receptor type 2 member 16,Fusion protein 2,exo-alpha-sialidase,Taste receptor type 2 member 16	polymer	1011	113855.8	1	UniProt (A0A4J1WX37) UniProt (Q9NYV7) Pfam (PF05296)	Homo sapiens (human)	T2R16
6	F (R)	2-(hydroxymethyl)phenyl beta-D-glucopyranoside	non-polymer		286.3	1	Chemie (SA0) PubChem (16757795) PubChem (5145) PubChem (21252322) PubChem (439503) PubChem (25791629) PubChem (25791630) PubChem (25791631) PubChem (40426983) PubChem (40468122) PubChem (40468123) PubChem (45933792) PubChem (44354315) PubChem (667502) PubChem (57997977) PubChem (70943559) PubChem (70943570) PubChem (71306773) PubChem (73265336) PubChem (6931410) PubChem (637126) PubChem (12315199) PubChem (12315200) PubChem (12315201) PubChem (154497406) PubChem (159771945) PubChem (173860252)

Sequence modifications

A: 6 - 64 (UniProt: P63092)

PDB	External Database	Details
Met -10	-	initiating methionine
Asp -9	-	expression tag
Tyr -8	-	expression tag
Lys -7	-	expression tag
Asp -6	-	expression tag
Asp -5	-	expression tag
Asp -4	-	expression tag
Asp -3	-	expression tag
Lys -2	-	expression tag
Glu -1	-	expression tag
Asn 0	-	expression tag
Leu 1	-	expression tag
Tyr 2	-	expression tag
Phe 3	-	expression tag
Gln 4	-	expression tag
Ser 5	-	expression tag
Asp 49	Gly 49	conflict
Asn 50	Glu 50	conflict
Gly 65	-	linker
Gly 66	-	linker
Ser 67	-	linker
Gly 67	-	linker
Gly 67	-	linker
Ser 67	-	linker
Gly 67	-	linker
Gly 67	-	linker

A: 67 - 249 (PDB: 9KPD)
B: 1 - 340 (UniProt: P62873)

PDB	External Database	Details
Gly 341	-	expression tag
Ser 342	-	expression tag
Ser 343	-	expression tag
Gly 344	-	expression tag
Gly 345	-	expression tag
Gly 346	-	expression tag
Gly 347	-	expression tag
Ser 348	-	expression tag
Gly 349	-	expression tag
Gly 350	-	expression tag
Gly 351	-	expression tag
Gly 352	-	expression tag
Ser 353	-	expression tag
Ser 354	-	expression tag
Gly 355	-	expression tag
Val 356	-	expression tag
Ser 357	-	expression tag
Gly 358	-	expression tag
Trp 359	-	expression tag
Arg 360	-	expression tag
Leu 361	-	expression tag
Phe 362	-	expression tag
Lys 363	-	expression tag
Lys 364	-	expression tag
Ile 365	-	expression tag
Ser 366	-	expression tag

R: -537 - -479 (PDB: 9KPD)
R: -478 - -4 (UniProt: A0A4J1WX37)

PDB	External Database	Details
Gly -3	-	linker
Ser -2	-	linker
Gly -1	-	linker
Ser 0	-	linker
Gly 1	-	linker

R: 2 - 291 (UniProt: Q9NYV7)

PDB	External Database	Details
Cys 133	Ser 133	conflict

R: 292 - 473 (PDB: 9KPD)

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	206874.2
Non-Polymers*	Number of molecules	1
	Total formula weight	286.3
All*	Total formula weight	207160.5

*Water molecules are not included.