9KPD

Cryo-EM structure of GPCR16-miniGs complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Guanine nucleotide-binding protein G(s) subunit alpha isoforms short,Guanine nucleotide-binding protein G(s) subunit alpha isoforms short,Guanine nucleotide-binding protein G(t) subunit alpha-3	polymer	264	30583.3	1	UniProt (P63092) Pfam (PF00503)	Homo sapiens (human)	Adenylate cyclase-stimulating G alpha protein
2	B (B)	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	366	39728.4	1	UniProt (P62873) Pfam (PF25391)	Homo sapiens (human)	Transducin beta chain 1
3	C (G)	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	polymer	71	7861.1	1	UniProt (P59768) Pfam (PF00631)	Homo sapiens (human)	G gamma-I
4	D (N)	Nanobody 35	polymer	135	14845.5	1		Homo sapiens (human)
5	E (R)	Fusion protein 1,exo-alpha-sialidase,Taste receptor type 2 member 16,Fusion protein 2,exo-alpha-sialidase,Taste receptor type 2 member 16	polymer	1011	113855.8	1	UniProt (A0A4J1WX37) UniProt (Q9NYV7) Pfam (PF05296)	Homo sapiens (human)	T2R16
6	F (R)	2-(hydroxymethyl)phenyl beta-D-glucopyranoside	non-polymer		286.3	1	Chemie (SA0)

Sequence modifications

A: 6 - 64 (UniProt: P63092)

PDB	External Database	Details
Met -10	-	initiating methionine
Asp -9	-	expression tag
Tyr -8	-	expression tag
Lys -7	-	expression tag
Asp -6	-	expression tag
Asp -5	-	expression tag
Asp -4	-	expression tag
Asp -3	-	expression tag
Lys -2	-	expression tag
Glu -1	-	expression tag
Asn 0	-	expression tag
Leu 1	-	expression tag
Tyr 2	-	expression tag
Phe 3	-	expression tag
Gln 4	-	expression tag
Ser 5	-	expression tag
Asp 49	Gly 49	conflict
Asn 50	Glu 50	conflict
Gly 65	-	linker
Gly 66	-	linker
Ser 67	-	linker
Gly 67	-	linker
Gly 67	-	linker
Ser 67	-	linker
Gly 67	-	linker
Gly 67	-	linker

A: 67 - 249 (PDB: 9KPD)
B: 1 - 340 (UniProt: P62873)

PDB	External Database	Details
Gly 341	-	expression tag
Ser 342	-	expression tag
Ser 343	-	expression tag
Gly 344	-	expression tag
Gly 345	-	expression tag
Gly 346	-	expression tag
Gly 347	-	expression tag
Ser 348	-	expression tag
Gly 349	-	expression tag
Gly 350	-	expression tag
Gly 351	-	expression tag
Gly 352	-	expression tag
Ser 353	-	expression tag
Ser 354	-	expression tag
Gly 355	-	expression tag
Val 356	-	expression tag
Ser 357	-	expression tag
Gly 358	-	expression tag
Trp 359	-	expression tag
Arg 360	-	expression tag
Leu 361	-	expression tag
Phe 362	-	expression tag
Lys 363	-	expression tag
Lys 364	-	expression tag
Ile 365	-	expression tag
Ser 366	-	expression tag

R: -537 - -479 (PDB: 9KPD)
R: -478 - -4 (UniProt: A0A4J1WX37)

PDB	External Database	Details
Gly -3	-	linker
Ser -2	-	linker
Gly -1	-	linker
Ser 0	-	linker
Gly 1	-	linker

R: 2 - 291 (UniProt: Q9NYV7)

PDB	External Database	Details
Cys 133	Ser 133	conflict

R: 292 - 473 (PDB: 9KPD)

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	206874.2
Non-Polymers*	Number of molecules	1
	Total formula weight	286.3
All*	Total formula weight	207160.5

*Water molecules are not included.