9KNW

Cryo-EM structure of apo human mitochondrial pyruvate carrier in the IMS-open conformation at pH 6.8

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (B)	Mitochondrial pyruvate carrier 2	polymer	151	17161.7	1	UniProt (O95563) Pfam (PF03650)	Homo sapiens (human)	Brain protein 44
2	B (A)	Mitochondrial pyruvate carrier 1	polymer	120	13592.8	1	UniProt (Q9Y5U8) Pfam (PF03650)	Homo sapiens (human)	Brain protein 44-like protein
3	C (C)	MPC specific nanobody 1	polymer	138	15255.8	1		Homo sapiens
4	D (B)	CARDIOLIPIN	non-polymer		1464.0	1	Chemie (CDL)
5	E (A)	1,2-DIOCTANOYL-SN-GLYCERO-3-PHOSPHOCHOLINE	non-polymer		510.6	1	Chemie (PC8)

Sequence modifications

B: 1 - 127 (UniProt: O95563)

PDB	External Database	Details
Gly 128	-	expression tag
Ser 129	-	expression tag
Asp 130	-	expression tag
Tyr 131	-	expression tag
Lys 132	-	expression tag
Asp 133	-	expression tag
His 134	-	expression tag
Asp 135	-	expression tag
Gly 136	-	expression tag
Asp 137	-	expression tag
Tyr 138	-	expression tag
Lys 139	-	expression tag
Asp 140	-	expression tag
His 141	-	expression tag
Asp 142	-	expression tag
Ile 143	-	expression tag
Asp 144	-	expression tag
Tyr 145	-	expression tag
Lys 146	-	expression tag
Asp 147	-	expression tag
Asp 148	-	expression tag
Asp 149	-	expression tag
Asp 150	-	expression tag
Lys 151	-	expression tag

A: 1 - 109 (UniProt: Q9Y5U8)

PDB	External Database	Details
Gly 110	-	expression tag
Ser 111	-	expression tag
Tyr 112	-	expression tag
Pro 113	-	expression tag
Tyr 114	-	expression tag
Asp 115	-	expression tag
Val 116	-	expression tag
Pro 117	-	expression tag
Asp 118	-	expression tag
Tyr 119	-	expression tag
Ala 120	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	46010.3
Non-Polymers*	Number of molecules	2
	Total formula weight	1974.7
All*	Total formula weight	47985.0

*Water molecules are not included.