9KMC
Cryo-EM structure of the heterotrimeric interleukin-2 receptor in complex with interleukin-2 and anti-CD25 Fab S417
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Interleukin-2 receptor subunit alpha | polymer | 240 | 26625.5 | 1 | UniProt (P01589) | Homo sapiens (human) | IL-2 receptor subunit alpha,IL-2-RA,IL-2R subunit alpha,IL2-RA,TAC antigen,p55 |
| 2 | B (B) | Interleukin-2 receptor subunit beta | polymer | 222 | 25824.2 | 1 | UniProt (P14784) | Homo sapiens (human) | IL-2 receptor subunit beta,IL-2R subunit beta,IL-2RB,High affinity IL-2 receptor subunit beta,Interleukin-15 receptor subunit beta,p70-75,p75 |
| 3 | C (C) | Cytokine receptor common subunit gamma | polymer | 241 | 28628.8 | 1 | UniProt (P31785) | Homo sapiens (human) | Interleukin-2 receptor subunit gamma,IL-2 receptor subunit gamma,IL-2R subunit gamma,IL-2RG,gammaC,p64 |
| 4 | D (D) | Interleukin-2 | polymer | 133 | 15436.0 | 1 | UniProt (P60568) | Homo sapiens (human) | IL-2,T-cell growth factor,TCGF |
| 5 | E (E) | Fab S417 heavy chain | polymer | 228 | 24459.4 | 1 | synthetic construct | ||
| 6 | F (F) | Fab S417 light chain | polymer | 213 | 23004.4 | 1 | synthetic construct | ||
| 7 | G, H (G, H) | 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-[alpha-L-fucopyranose-(1-6)]2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 570.5 | 2 | In PDB GlyTouCan (G21290RB) | |||
| 8 | I (I) | beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 586.5 | 1 | In PDB GlyTouCan (G15407YE) | |||
| 9 | J, K (J, K) | 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 424.4 | 2 | In PDB GlyTouCan (G42666HT) | |||
| 10 | L (C) | 2-acetamido-2-deoxy-beta-D-glucopyranose | non-polymer | 221.2 | 1 | Chemie (NAG) |
Sequence modifications
A: 22 - 240 (UniProt: P01589)
B: 27 - 240 (UniProt: P14784)
C: 23 - 255 (UniProt: P31785)
| PDB | External Database | Details |
|---|---|---|
| Gly 241 | - | expression tag |
| Gly 242 | - | expression tag |
| Gly 243 | - | expression tag |
| Gly 244 | - | expression tag |
| Ser 245 | - | expression tag |
| Gly 246 | - | expression tag |
| Gly 247 | - | expression tag |
| Gly 248 | - | expression tag |
| Gly 249 | - | expression tag |
| Ser 250 | - | expression tag |
| Gly 251 | - | expression tag |
| Gly 252 | - | expression tag |
| Gly 253 | - | expression tag |
| Gly 254 | - | expression tag |
| Ser 255 | - | expression tag |
| His 256 | - | expression tag |
| His 257 | - | expression tag |
| His 258 | - | expression tag |
| His 259 | - | expression tag |
| His 260 | - | expression tag |
| His 261 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| His 241 | - | expression tag |
| His 242 | - | expression tag |
| His 243 | - | expression tag |
| His 244 | - | expression tag |
| His 245 | - | expression tag |
| His 246 | - | expression tag |
| His 247 | - | expression tag |
| His 248 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Gln 75 | Asn 75 | conflict |
| His 256 | - | expression tag |
| His 257 | - | expression tag |
| His 258 | - | expression tag |
| His 259 | - | expression tag |
| His 260 | - | expression tag |
| His 261 | - | expression tag |
| His 262 | - | expression tag |
| His 263 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 6 |
| Total formula weight | 143978.3 | |
| Branched | Number of molecules | 5 |
| Total formula weight | 2576.4 | |
| Non-Polymers* | Number of molecules | 1 |
| Total formula weight | 221.2 | |
| All* | Total formula weight | 146776.0 |
