9KLO
Cryo-EM structure of ChCas12b-sgRNA-extended non-target DNA ternary complex (Complex-B)
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | C2c1 CRISPR-Cas endonuclease RuvC-like domain-containing protein | polymer | 1496 | 167419.8 | 1 | UniProt (A0A1V5YSD0) | Candidatus Hydrogenedentes bacterium ADurb.Bin170 | |
| 2 | B (B) | sgRNA | polymer | 115 | 37052.0 | 1 | Candidatus Hydrogenedentes bacterium ADurb.Bin170 | ||
| 3 | C (C) | Target DNA strand | polymer | 51 | 15570.0 | 1 | Candidatus Hydrogenedentes bacterium ADurb.Bin170 | ||
| 4 | D (D) | Non-target DNA strand | polymer | 51 | 15684.0 | 1 | Candidatus Hydrogenedentes bacterium ADurb.Bin170 | ||
| 5 | E, F, G, H (A, B) | CALCIUM ION | non-polymer | 40.1 | 4 | Chemie (CA) | |||
| 6 | I, J (A, D) | water | water | 18.0 | 4 | Chemie (HOH) |
Sequence modifications
A: 2 - 1496 (UniProt: A0A1V5YSD0)
| PDB | External Database | Details |
|---|---|---|
| Pro 1 | - | expression tag |
| Ala 496 | Asp 496 | conflict |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 4 |
| Total formula weight | 235725.8 | |
| Non-Polymers* | Number of molecules | 4 |
| Total formula weight | 160.3 | |
| All* | Total formula weight | 235886.1 |
