9KLN
Cryo-EM structure of ChCas12b-sgRNA-target DNA ternary complex (Complex-A)
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | C2c1 CRISPR-Cas endonuclease RuvC-like domain-containing protein | polymer | 1496 | 167419.8 | 1 | UniProt (A0A1V5YSD0) | Candidatus Hydrogenedentes bacterium ADurb.Bin170 | |
| 2 | B (B) | sgRNA | polymer | 115 | 37052.0 | 1 | Candidatus Hydrogenedentes bacterium ADurb.Bin170 | ||
| 3 | C (C) | Target DNA strand | polymer | 33 | 10080.5 | 1 | Candidatus Hydrogenedentes bacterium ADurb.Bin170 | ||
| 4 | D (D) | Non-target DNA strand | polymer | 9 | 2746.8 | 1 | Candidatus Hydrogenedentes bacterium ADurb.Bin170 | ||
| 5 | E, F (B) | MAGNESIUM ION | non-polymer | 24.3 | 2 | Chemie (MG) | |||
| 6 | G (B) | water | water | 18.0 | 8 | Chemie (HOH) |
Sequence modifications
A: 2 - 1496 (UniProt: A0A1V5YSD0)
| PDB | External Database | Details |
|---|---|---|
| Pro 1 | - | expression tag |
| Ala 496 | Asp 496 | conflict |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 4 |
| Total formula weight | 217299.2 | |
| Non-Polymers* | Number of molecules | 2 |
| Total formula weight | 48.6 | |
| All* | Total formula weight | 217347.8 |
