9KKY
Co-crystal structure of human 8-oxoguanine glycosylase N149C mutant with DNA containing photocaged 8-oxoguanine
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (B) | DNA (5'-D(P*GP*GP*TP*AP*GP*AP*CP*CP*TP*GP*G)-3') | polymer | 16 | 4939.2 | 1 | Homo sapiens | ||
| 2 | B (C) | DNA (5'-D(*AP*(8OG)P*GP*TP*CP*TP*AP*C)-3') | polymer | 16 | 4875.2 | 1 | Homo sapiens | ||
| 3 | C (A) | N-glycosylase/DNA lyase | polymer | 337 | 37910.9 | 1 | UniProt (O15527) Pfam (PF07934) Pfam (PF00730) | Homo sapiens (human) | |
| 4 | D, F, G, H (A) | CALCIUM ION | non-polymer | 40.1 | 4 | Chemie (CA) | |||
| 5 | E (A) | GLYCEROL | non-polymer | 92.1 | 1 | Chemie (GOL) | |||
| 6 | I, J (C, A) | water | water | 18.0 | 49 | Chemie (HOH) |
Sequence modifications
A: 11 - 327 (UniProt: O15527)
| PDB | External Database | Details |
|---|---|---|
| Met -9 | - | initiating methionine |
| Gly -8 | - | expression tag |
| Ser -7 | - | expression tag |
| Ser -6 | - | expression tag |
| His -5 | - | expression tag |
| His -4 | - | expression tag |
| His -3 | - | expression tag |
| His -2 | - | expression tag |
| His -1 | - | expression tag |
| His 0 | - | expression tag |
| Ser 1 | - | expression tag |
| Ser 2 | - | expression tag |
| Gly 3 | - | expression tag |
| Leu 4 | - | expression tag |
| Val 5 | - | expression tag |
| Pro 6 | - | expression tag |
| Arg 7 | - | expression tag |
| Gly 8 | - | expression tag |
| Ser 9 | - | expression tag |
| His 10 | - | expression tag |
| Cys 149 | Asn 149 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 3 |
| Total formula weight | 47725.3 | |
| Non-Polymers* | Number of molecules | 5 |
| Total formula weight | 252.4 | |
| All* | Total formula weight | 47977.7 |
