9KKI

Crystal structure of the CYP153A double mutant L354V/V456G from Marinobacter aquaeolei

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Cytochrome P450	polymer	480	55222.9	2	UniProt (A0A368UNN3) Pfam (PF00067)	Marinobacter nauticus (Marinobacter hydrocarbonoclasticus Marinobacter aquaeolei)
2	C, E (A, B)	PROTOPORPHYRIN IX CONTAINING FE	non-polymer		616.5	2	Chemie (HEM)
3	D, F (A, B)	LAURIC ACID	non-polymer		200.3	2	Chemie (DAO)
4	G, H (A, B)	water	water		18.0	471	Chemie (HOH)

Sequence modifications

A, B: 5 - 474 (UniProt: A0A368UNN3)

PDB	External Database	Details
Met 3	-	initiating methionine
Gly 4	-	expression tag
Val 358	Leu 354	engineered mutation
Gly 460	Val 456	engineered mutation
Leu 475	-	expression tag
Glu 476	-	expression tag
His 477	-	expression tag
His 478	-	expression tag
His 479	-	expression tag
His 480	-	expression tag
His 481	-	expression tag
His 482	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	110445.9
Non-Polymers*	Number of molecules	4
	Total formula weight	1633.6
All*	Total formula weight	112079.5

*Water molecules are not included.