9KJ6
Crystal Structure of SpCas9 ternary complex, amino acids (1242-1263) replaced with Gly-Ser linker
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | CRISPR-associated endonuclease Cas9/Csn1 | polymer | 1357 | 156702.6 | 1 | UniProt (Q99ZW2) Pfam (PF16593) Pfam (PF16592) Pfam (PF13395) Pfam (PF22702) Pfam (PF16595) | Streptococcus pyogenes | SpCas9,SpyCas9 |
| 2 | B (B) | single-guide RNA | polymer | 102 | 32991.6 | 1 | Streptococcus pyogenes | ||
| 3 | C (C) | Target Strand of double-stranded DNA | polymer | 43 | 13126.4 | 1 | synthetic construct | ||
| 4 | D (D) | Non-target Strand of double-stranded DNA | polymer | 27 | 8349.4 | 1 | synthetic construct |
Sequence modifications
A: 1 - 1241 (UniProt: Q99ZW2)
A: 1249 - 1353 (UniProt: Q99ZW2)
| PDB | External Database | Details |
|---|---|---|
| Gly -3 | - | expression tag |
| Ala -2 | - | expression tag |
| Ala -1 | - | expression tag |
| Ser 0 | - | expression tag |
| Ala 10 | Asp 10 | engineered mutation |
| Ala 840 | His 840 | engineered mutation |
| PDB | External Database | Details |
|---|---|---|
| Gly 1242 | - | linker |
| Ser 1243 | - | linker |
| Gly 1244 | - | linker |
| Ser 1245 | - | linker |
| Gly 1246 | - | linker |
| Ser 1247 | - | linker |
| Gly 1248 | - | linker |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 4 |
| Total formula weight | 211170.0 | |
| All* | Total formula weight | 211170.0 |
