9KHT
cryo-EM structure of Ufd2/Ubc4-Ub in complex with K29-linked triUb (dimeric conformation)
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, D (D, d) | Polyubiquitin-C | polymer | 76 | 8550.8 | 2 | UniProt (P0CG48) Pfam (PF00240) | Homo sapiens (human) | |
| 2 | B, C, E, F, G... (E, F, e, f, C...) | Ubiquitin | polymer | 76 | 8576.8 | 6 | UniProt (P62979) Pfam (PF00240) | Homo sapiens (human) | |
| 3 | I, K (A, a) | E4 ubiquitin-protein ligase UFD2 | polymer | 955 | 109360.7 | 2 | UniProt (P54860) Pfam (PF10408) Pfam (PF04564) | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) | RING-type E3 ubiquitin transferase UFD2,Ubiquitin conjugation factor E4,Ubiquitin fusion degradation protein 2,UB fusion protein 2 |
| 4 | J, L (B, b) | Ubiquitin-conjugating enzyme E2 4 | polymer | 148 | 16442.6 | 2 | UniProt (P15731) Pfam (PF00179) | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) | E2 ubiquitin-conjugating enzyme 4,Ubiquitin carrier protein 4,Ubiquitin-protein ligase 4 |
Sequence modifications
D, d: 1 - 76 (UniProt: P0CG48)
B, b: 1 - 148 (UniProt: P15731)
| PDB | External Database | Details |
|---|---|---|
| Cys 48 | Lys 48 | engineered mutation |
| PDB | External Database | Details |
|---|---|---|
| Ser 22 | Cys 22 | engineered mutation |
| Ser 108 | Cys 108 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 12 |
| Total formula weight | 320169.2 | |
| All* | Total formula weight | 320169.2 |
