9KG5

Crystal structure of the CYP154C5 F92A/R114A/T248D/E282A variant from Nocardia farcinica

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (B, A)	Cytochrome P450 monooxygenase	polymer	429	47184.7	2	UniProt (Q5YNS8) Pfam (PF00067)	Nocardia farcinica IFM 10152
2	C, K (B, A)	TESTOSTERONE	non-polymer		288.4	2	Chemie (TES)
3	D, L (B, A)	PROTOPORPHYRIN IX CONTAINING FE	non-polymer		616.5	2	Chemie (HEM)
4	E, F, G, N, O (B, A)	CADMIUM ION	non-polymer		112.4	5	Chemie (CD)
5	H, P (B, A)	CHLORIDE ION	non-polymer		35.5	2	Chemie (CL)
6	I, J (B)	POTASSIUM ION	non-polymer		39.1	2	Chemie (K)
7	M (A)	GLYCEROL	non-polymer		92.1	1	Chemie (GOL)
8	Q, R (B, A)	water	water		18.0	757	Chemie (HOH)

Sequence modifications

B, A: 1 - 409 (UniProt: Q5YNS8)

PDB	External Database	Details
Met -19	-	initiating methionine
Gly -18	-	expression tag
Ser -17	-	expression tag
Ser -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
Ser -9	-	expression tag
Ser -8	-	expression tag
Gly -7	-	expression tag
Leu -6	-	expression tag
Val -5	-	expression tag
Pro -4	-	expression tag
Arg -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
His 0	-	expression tag
Ala 92	Phe 92	engineered mutation
Ala 114	Arg 114	engineered mutation
Asp 248	Thr 248	engineered mutation
Ala 282	Glu 282	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	94369.4
Non-Polymers*	Number of molecules	14
	Total formula weight	2613.1
All*	Total formula weight	96982.5

*Water molecules are not included.