9KFK

Cryo-EM structure of the relaxin-3-bound human relaxin family peptide receptor 4 (RXFP4)-Gi complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (G)	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	polymer	55	6089.0	1	UniProt (P63212) Pfam (PF00631)	Bos taurus (domestic cattle)	G gamma-I
2	B (I)	Guanine nucleotide-binding protein G(i) subunit alpha-2	polymer	355	40502.9	1	UniProt (P04899) Pfam (PF00503)	Homo sapiens (human)	Adenylate cyclase-inhibiting G alpha protein
3	C (R)	Soluble cytochrome b562,Relaxin-3 receptor 2,LgBiT	polymer	678	74611.0	1	UniProt (P0ABE7) UniProt (Q8TDU9) Pfam (PF00001)	Escherichia coli	Cytochrome b-562,RLN3 receptor 2,G-protein coupled receptor 100,G-protein coupled receptor GPCR142,Insulin-like peptide INSL5 receptor,Relaxin family peptide receptor 4
4	D (S)	scFv16	polymer	248	26408.5	1		synthetic construct
5	E (T)	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	338	37198.7	1	UniProt (P62873) Pfam (PF00400)	Homo sapiens (human)	Transducin beta chain 1
6	F (B)	Relaxin-3 B chain	polymer	19	2174.6	1	UniProt (Q8WXF3)	Homo sapiens (human)
7	G (A)	Relaxin-3 A chain	polymer	24	2465.8	1	UniProt (Q8WXF3)	Homo sapiens (human)

Sequence modifications

I: 1 - 355 (UniProt: P04899)

PDB	External Database	Details
Asn 47	Ser 47	engineered mutation
Ala 204	Gly 204	engineered mutation
Ala 246	Glu 246	engineered mutation
Ser 327	Ala 327	engineered mutation

R: -104 - 1 (UniProt: P0ABE7)

PDB	External Database	Details
Met -130	-	initiating methionine
Lys -129	-	expression tag
Thr -128	-	expression tag
Ile -127	-	expression tag
Ile -126	-	expression tag
Ala -125	-	expression tag
Leu -124	-	expression tag
Ser -123	-	expression tag
Tyr -122	-	expression tag
Ile -121	-	expression tag
Phe -120	-	expression tag
Cys -119	-	expression tag
Leu -118	-	expression tag
Val -117	-	expression tag
Phe -116	-	expression tag
Ala -115	-	expression tag
His -114	-	expression tag
His -113	-	expression tag
His -112	-	expression tag
His -111	-	expression tag
His -110	-	expression tag
His -109	-	expression tag
His -108	-	expression tag
His -107	-	expression tag
His -106	-	expression tag
His -105	-	expression tag
Trp -98	Met 29	engineered mutation
Ile -3	His 124	engineered mutation
Leu 1	Arg 128	engineered mutation

R: 2 - 374 (UniProt: Q8TDU9)
R: 375 - 547 (PDB: 9KFK)

Contents of the asymmetric unit

Polymers	Number of chains	7
	Total formula weight	189450.5
All*	Total formula weight	189450.5

*Water molecules are not included.