9KFJ

Cryo-EM structure of the compound 4-bound human relaxin family peptide receptor 3 (RXFP3)-Gi complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (C)	Soluble cytochrome b562,Relaxin-3 receptor 1,LgBiT	polymer	773	84607.5	1	UniProt (P0ABE7) UniProt (Q9NSD7) Pfam (PF00001)	Escherichia coli	Cytochrome b-562,RLN3 receptor 1,G protein-coupled receptor SALPR,G-protein coupled receptor GPCR135,Relaxin family peptide receptor 3,Somatostatin- and angiotensin-like peptide receptor
2	B (G)	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	polymer	55	6089.0	1	UniProt (P59768) Pfam (PF00631)	Homo sapiens (human)	G gamma-I
3	C (I)	Guanine nucleotide-binding protein G(i) subunit alpha-2	polymer	355	40502.9	1	UniProt (P04899) Pfam (PF00503)	Homo sapiens (human)	Adenylate cyclase-inhibiting G alpha protein
4	D (S)	scFv16	polymer	247	26277.3	1		synthetic construct
5	E (T)	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	338	37198.7	1	UniProt (P62873) Pfam (PF00400)	Homo sapiens (human)	Transducin beta chain 1
6	F (C)	1-[2-(4-chlorophenyl)ethyl]-3-[(7-ethyl-5-oxidanyl-1H-indol-3-yl)methylideneamino]guanidine	non-polymer		383.9	1	Chemie (IYF)

Sequence modifications

C: -104 - 1 (UniProt: P0ABE7)

PDB	External Database	Details
Met -130	-	initiating methionine
Lys -129	-	expression tag
Thr -128	-	expression tag
Ile -127	-	expression tag
Ile -126	-	expression tag
Ala -125	-	expression tag
Leu -124	-	expression tag
Ser -123	-	expression tag
Tyr -122	-	expression tag
Ile -121	-	expression tag
Phe -120	-	expression tag
Cys -119	-	expression tag
Leu -118	-	expression tag
Val -117	-	expression tag
Phe -116	-	expression tag
Ala -115	-	expression tag
His -114	-	expression tag
His -113	-	expression tag
His -112	-	expression tag
His -111	-	expression tag
His -110	-	expression tag
His -109	-	expression tag
His -108	-	expression tag
His -107	-	expression tag
His -106	-	expression tag
His -105	-	expression tag
Trp -98	Met 29	engineered mutation
Ile -3	His 124	engineered mutation
Leu 1	Arg 128	engineered mutation

C: 2 - 469 (UniProt: Q9NSD7)
C: 470 - 642 (PDB: 9KFJ)
I: 1 - 355 (UniProt: P04899)

PDB	External Database	Details
Asn 47	Ser 47	engineered mutation
Ala 204	Gly 204	engineered mutation
Ala 246	Glu 246	engineered mutation
Ser 327	Ala 327	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	194675.3
Non-Polymers*	Number of molecules	1
	Total formula weight	383.9
All*	Total formula weight	195059.2

*Water molecules are not included.