9KE3

Cryo-EM structure of lipid-mediated dimer of human norepinephrine transporter NET in the presence of the F3288-0031 in an inward-open state at resolution of 3.1 angstrom

This is a non-PDB format compatible entry.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Sodium-dependent noradrenaline transporter	polymer	629	70715.9	2	UniProt (P23975) Pfam (PF00209)	Homo sapiens (human)	Norepinephrine transporter,NET,Solute carrier family 6 member 2
2	C, R (A, B)	(2~{S})-1-[2-methoxy-4-[(~{E})-prop-1-enyl]phenoxy]-3-[4-(phenylmethyl)piperazin-1-yl]propan-2-ol	non-polymer		396.5	2	Chemie (A1EFR)
3	D, E, F, G, H... (A, B)	CHOLESTEROL	non-polymer		386.7	14	Chemie (CLR)
4	K, Q (A, B)	[(2~{R})-1-[[(2~{S})-2,3-bis(oxidanyl)propoxy]-oxidanyl-phosphoryl]oxy-3-nonanoyloxy-propan-2-yl] decanoate	non-polymer		540.6	2	Chemie (A1EFM)

Sequence modifications

A, B: 1 - 617 (UniProt: P23975)

PDB	External Database	Details
Met -11	-	initiating methionine
Asp -10	-	expression tag
Tyr -9	-	expression tag
Lys -8	-	expression tag
Asp -7	-	expression tag
Asp -6	-	expression tag
Asp -5	-	expression tag
Asp -4	-	expression tag
Lys -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
Gly 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	141431.8
Non-Polymers*	Number of molecules	18
	Total formula weight	7287.4
All*	Total formula weight	148719.2

*Water molecules are not included.