9KB9

Cryo-EM structure of LGR4-RSPO2-ZNRF3 complex (2:2:2)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, D (A, D)	Leucine-rich repeat-containing G-protein coupled receptor 4	polymer	845	93372.0	2	UniProt (Q9BXB1)	Homo sapiens (human)	G-protein coupled receptor 48
2	B, E (C, E)	E3 ubiquitin-protein ligase ZNRF3	polymer	231	25888.1	2	UniProt (Q9ULT6)	Homo sapiens (human)	RING finger protein 203,RING-type E3 ubiquitin transferase ZNRF3,Zinc/RING finger protein 3
3	C, F (B, F)	R-spondin-2	polymer	160	18953.7	2	UniProt (Q6UXX9)	Homo sapiens (human)	Roof plate-specific spondin-2,hRspo2
4	G, H, I, J (A, D)	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	4	Chemie (NAG)

Sequence modifications

A, D: 1 - 822 (UniProt: Q9BXB1)

PDB	External Database	Details
Glu 823	-	expression tag
Asn 824	-	expression tag
Leu 825	-	expression tag
Tyr 826	-	expression tag
Phe 827	-	expression tag
Gln 828	-	expression tag
Gly 829	-	expression tag
Asp 830	-	expression tag
Tyr 831	-	expression tag
Lys 832	-	expression tag
Asp 833	-	expression tag
Asp 834	-	expression tag
Asp 835	-	expression tag
Asp 836	-	expression tag
Lys 837	-	expression tag
His 838	-	expression tag
His 839	-	expression tag
His 840	-	expression tag
His 841	-	expression tag
His 842	-	expression tag
His 843	-	expression tag
His 844	-	expression tag
His 845	-	expression tag

C, E: 56 - 267 (UniProt: Q9ULT6)

PDB	External Database	Details
Met 37	-	initiating methionine
Glu 38	-	expression tag
Thr 39	-	expression tag
Gly 40	-	expression tag
Leu 41	-	expression tag
Arg 42	-	expression tag
Trp 43	-	expression tag
Leu 44	-	expression tag
Leu 45	-	expression tag
Leu 46	-	expression tag
Val 47	-	expression tag
Ala 48	-	expression tag
Val 49	-	expression tag
Leu 50	-	expression tag
Lys 51	-	expression tag
Gly 52	-	expression tag
Val 53	-	expression tag
Gln 54	-	expression tag
Cys 55	-	expression tag

B, F: 1 - 143 (UniProt: Q6UXX9)

PDB	External Database	Details
Glu 144	-	expression tag
Asn 145	-	expression tag
Leu 146	-	expression tag
Tyr 147	-	expression tag
Phe 148	-	expression tag
Gln 149	-	expression tag
Gly 150	-	expression tag
His 151	-	expression tag
His 152	-	expression tag
His 153	-	expression tag
His 154	-	expression tag
His 155	-	expression tag
His 156	-	expression tag
His 157	-	expression tag
His 158	-	expression tag
His 159	-	expression tag
His 160	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	276427.7
Non-Polymers*	Number of molecules	4
	Total formula weight	884.8
All*	Total formula weight	277312.5

*Water molecules are not included.