9K8X

Crystal structure of the calcium indicator GCaMP6s-BrUS-145 in calcium-bounded state

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Calcium indicator GCaMP6s-BrUS-145,Calmodulin-1	polymer	462	51900.9	1	UniProt (P0DP23) Pfam (PF01353) Pfam (PF13499) UniProt (by SIFTS) (P42212)	synthetic construct
2	B, C, D, E, F... (A)	1,2-ETHANEDIOL	non-polymer		62.1	6	Chemie (EDO)
3	H, I, J, K (A)	CALCIUM ION	non-polymer		40.1	4	Chemie (CA)
4	L (A)	SODIUM ION	non-polymer		23.0	1	Chemie (NA)
5	M (A)	water	water		18.0	286	Chemie (HOH)

Sequence modifications

A: 1 - 314 (PDB: 9K8X)
A: 315 - 462 (UniProt: P0DP23)

PDB	External Database	Details
Pro 315	Ala 2	engineered mutation
Asp 374	Asn 61	engineered mutation
Tyr 392	Asp 79	engineered mutation
Arg 393	Thr 80	engineered mutation
Thr 395	Ser 82	engineered mutation
Gly 404	Arg 91	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	51900.9
Non-Polymers*	Number of molecules	11
	Total formula weight	555.7
All*	Total formula weight	52456.6

*Water molecules are not included.