9K7C
Crystal structure of GH57 family amylopullulanase from Aquifex aeolicus mutant E256Q in complex with maltopentaose
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B, C, D (A, B, C, D) | Glycoside hydrolase family 57 N-terminal domain-containing protein | polymer | 477 | 57252.3 | 4 | UniProt (O66934) Pfam (PF03065) | Aquifex aeolicus VF5 | |
| 2 | E, I (E, I) | alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose | branched | 504.4 | 2 | In PDB BIRD (PRD_900009) GlyTouCan (G96370VA) | |||
| 3 | F, H, J, K (F, H, J, K) | alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose | branched | 828.7 | 4 | In PDB GlyTouCan (G50146AM) | |||
| 4 | G (G) | alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose | branched | 666.6 | 1 | In PDB GlyTouCan (G87171PZ) | |||
| 5 | L (B) | GLYCEROL | non-polymer | 92.1 | 1 | Chemie (GOL) | |||
| 6 | M, N, O, P (A, B, C, D) | water | water | 18.0 | 1007 | Chemie (HOH) |
Sequence modifications
A, B, C, D: 1 - 477 (UniProt: O66934)
| PDB | External Database | Details |
|---|---|---|
| Gln 256 | Glu 256 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 4 |
| Total formula weight | 229009.0 | |
| Branched | Number of molecules | 7 |
| Total formula weight | 4990.3 | |
| Non-Polymers* | Number of molecules | 1 |
| Total formula weight | 92.1 | |
| All* | Total formula weight | 234091.4 |
