9K6L
Cryo-EM structure of GPCR16-Gi2 complex
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Guanine nucleotide-binding protein G(i) subunit alpha-2 | polymer | 355 | 40560.9 | 1 | UniProt (P04899) Pfam (PF00503) | Homo sapiens (human) | Adenylate cyclase-inhibiting G alpha protein |
| 2 | B (B) | Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 | polymer | 366 | 39728.4 | 1 | UniProt (P62873) Pfam (PF25391) | Homo sapiens (human) | Transducin beta chain 1 |
| 3 | C (G) | Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 | polymer | 71 | 7861.1 | 1 | UniProt (P59768) Pfam (PF00631) | Homo sapiens (human) | G gamma-I |
| 4 | D (S) | scFv16 | polymer | 266 | 28666.8 | 1 | Homo sapiens | ||
| 5 | E (R) | exo-alpha-sialidase,Taste receptor type 2 member 16,LgBiT | polymer | 1011 | 113855.8 | 1 | UniProt (A0A4J2AMT3) UniProt (Q9NYV7) Pfam (PF05296) | Streptococcus pneumoniae | Neuraminidase A,T2R16 |
| 6 | F (R) | 2-(hydroxymethyl)phenyl beta-D-glucopyranoside | non-polymer | 286.3 | 1 | Chemie (SA0) PubChem (16757795) PubChem (5145) PubChem (21252322) PubChem (439503) PubChem (25791629) PubChem (25791630) PubChem (25791631) PubChem (40426983) PubChem (40468122) PubChem (40468123) PubChem (45933792) PubChem (44354315) PubChem (667502) PubChem (57997977) PubChem (70943559) PubChem (70943570) PubChem (71306773) PubChem (73265336) PubChem (6931410) PubChem (637126) PubChem (12315199) PubChem (12315200) PubChem (12315201) PubChem (154497406) PubChem (159771945) PubChem (173860252) |
Sequence modifications
A: 1 - 355 (UniProt: P04899)
B: 1 - 340 (UniProt: P62873)
R: -478 - -4 (UniProt: A0A4J2AMT3)
R: 2 - 291 (UniProt: Q9NYV7)
R: 292 - 473 (PDB: 9K6L)
| PDB | External Database | Details |
|---|---|---|
| Asn 47 | Ser 47 | engineered mutation |
| Ala 204 | Gly 204 | engineered mutation |
| Ser 327 | Ala 327 | engineered mutation |
| PDB | External Database | Details |
|---|---|---|
| Gly 341 | - | expression tag |
| Ser 342 | - | expression tag |
| Ser 343 | - | expression tag |
| Gly 344 | - | expression tag |
| Gly 345 | - | expression tag |
| Gly 346 | - | expression tag |
| Gly 347 | - | expression tag |
| Ser 348 | - | expression tag |
| Gly 349 | - | expression tag |
| Gly 350 | - | expression tag |
| Gly 351 | - | expression tag |
| Gly 352 | - | expression tag |
| Ser 353 | - | expression tag |
| Ser 354 | - | expression tag |
| Gly 355 | - | expression tag |
| Val 356 | - | expression tag |
| Ser 357 | - | expression tag |
| Gly 358 | - | expression tag |
| Trp 359 | - | expression tag |
| Arg 360 | - | expression tag |
| Leu 361 | - | expression tag |
| Phe 362 | - | expression tag |
| Lys 363 | - | expression tag |
| Lys 364 | - | expression tag |
| Ile 365 | - | expression tag |
| Ser 366 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Met -537 | - | initiating methionine |
| Lys -536 | - | expression tag |
| Thr -535 | - | expression tag |
| Ile -534 | - | expression tag |
| Ile -533 | - | expression tag |
| Ala -532 | - | expression tag |
| Leu -531 | - | expression tag |
| Ser -530 | - | expression tag |
| Tyr -529 | - | expression tag |
| Ile -528 | - | expression tag |
| Phe -527 | - | expression tag |
| Cys -526 | - | expression tag |
| Leu -525 | - | expression tag |
| Val -524 | - | expression tag |
| Phe -523 | - | expression tag |
| Ala -522 | - | expression tag |
| Asp -521 | - | expression tag |
| Tyr -520 | - | expression tag |
| Lys -519 | - | expression tag |
| Asp -518 | - | expression tag |
| Asp -517 | - | expression tag |
| Asp -516 | - | expression tag |
| Asp -515 | - | expression tag |
| Ala -514 | - | expression tag |
| His -513 | - | expression tag |
| His -512 | - | expression tag |
| His -511 | - | expression tag |
| His -510 | - | expression tag |
| His -509 | - | expression tag |
| His -508 | - | expression tag |
| His -507 | - | expression tag |
| His -506 | - | expression tag |
| His -505 | - | expression tag |
| His -504 | - | expression tag |
| Glu -503 | - | expression tag |
| Asn -502 | - | expression tag |
| Leu -501 | - | expression tag |
| Tyr -500 | - | expression tag |
| Phe -499 | - | expression tag |
| Gln -498 | - | expression tag |
| Ser -497 | - | expression tag |
| Ala -496 | - | expression tag |
| His -495 | - | expression tag |
| His -494 | - | expression tag |
| His -493 | - | expression tag |
| His -492 | - | expression tag |
| His -491 | - | expression tag |
| His -490 | - | expression tag |
| Ser -489 | - | expression tag |
| Ser -488 | - | expression tag |
| Gly -487 | - | expression tag |
| Leu -486 | - | expression tag |
| Glu -485 | - | expression tag |
| Val -484 | - | expression tag |
| Leu -483 | - | expression tag |
| Phe -482 | - | expression tag |
| Gln -481 | - | expression tag |
| Gly -480 | - | expression tag |
| Pro -479 | - | expression tag |
| Gly -3 | - | linker |
| Ser -2 | - | linker |
| Gly -1 | - | linker |
| Ser 0 | - | linker |
| Gly 1 | - | linker |
| PDB | External Database | Details |
|---|---|---|
| Cys 133 | Ser 133 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 5 |
| Total formula weight | 230673.0 | |
| Non-Polymers* | Number of molecules | 1 |
| Total formula weight | 286.3 | |
| All* | Total formula weight | 230959.3 |
