9K6L

Cryo-EM structure of GPCR16-Gi2 complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Guanine nucleotide-binding protein G(i) subunit alpha-2	polymer	355	40560.9	1	UniProt (P04899) Pfam (PF00503)	Homo sapiens (human)	Adenylate cyclase-inhibiting G alpha protein
2	B (B)	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	366	39728.4	1	UniProt (P62873) Pfam (PF25391)	Homo sapiens (human)	Transducin beta chain 1
3	C (G)	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	polymer	71	7861.1	1	UniProt (P59768) Pfam (PF00631)	Homo sapiens (human)	G gamma-I
4	D (S)	scFv16	polymer	266	28666.8	1		Homo sapiens
5	E (R)	exo-alpha-sialidase,Taste receptor type 2 member 16,LgBiT	polymer	1011	113855.8	1	UniProt (A0A4J2AMT3) UniProt (Q9NYV7) Pfam (PF05296)	Streptococcus pneumoniae	Neuraminidase A,T2R16
6	F (R)	2-(hydroxymethyl)phenyl beta-D-glucopyranoside	non-polymer		286.3	1	Chemie (SA0)

Sequence modifications

A: 1 - 355 (UniProt: P04899)

PDB	External Database	Details
Asn 47	Ser 47	engineered mutation
Ala 204	Gly 204	engineered mutation
Ser 327	Ala 327	engineered mutation

B: 1 - 340 (UniProt: P62873)

PDB	External Database	Details
Gly 341	-	expression tag
Ser 342	-	expression tag
Ser 343	-	expression tag
Gly 344	-	expression tag
Gly 345	-	expression tag
Gly 346	-	expression tag
Gly 347	-	expression tag
Ser 348	-	expression tag
Gly 349	-	expression tag
Gly 350	-	expression tag
Gly 351	-	expression tag
Gly 352	-	expression tag
Ser 353	-	expression tag
Ser 354	-	expression tag
Gly 355	-	expression tag
Val 356	-	expression tag
Ser 357	-	expression tag
Gly 358	-	expression tag
Trp 359	-	expression tag
Arg 360	-	expression tag
Leu 361	-	expression tag
Phe 362	-	expression tag
Lys 363	-	expression tag
Lys 364	-	expression tag
Ile 365	-	expression tag
Ser 366	-	expression tag

R: -478 - -4 (UniProt: A0A4J2AMT3)

PDB	External Database	Details
Met -537	-	initiating methionine
Lys -536	-	expression tag
Thr -535	-	expression tag
Ile -534	-	expression tag
Ile -533	-	expression tag
Ala -532	-	expression tag
Leu -531	-	expression tag
Ser -530	-	expression tag
Tyr -529	-	expression tag
Ile -528	-	expression tag
Phe -527	-	expression tag
Cys -526	-	expression tag
Leu -525	-	expression tag
Val -524	-	expression tag
Phe -523	-	expression tag
Ala -522	-	expression tag
Asp -521	-	expression tag
Tyr -520	-	expression tag
Lys -519	-	expression tag
Asp -518	-	expression tag
Asp -517	-	expression tag
Asp -516	-	expression tag
Asp -515	-	expression tag
Ala -514	-	expression tag
His -513	-	expression tag
His -512	-	expression tag
His -511	-	expression tag
His -510	-	expression tag
His -509	-	expression tag
His -508	-	expression tag
His -507	-	expression tag
His -506	-	expression tag
His -505	-	expression tag
His -504	-	expression tag
Glu -503	-	expression tag
Asn -502	-	expression tag
Leu -501	-	expression tag
Tyr -500	-	expression tag
Phe -499	-	expression tag
Gln -498	-	expression tag
Ser -497	-	expression tag
Ala -496	-	expression tag
His -495	-	expression tag
His -494	-	expression tag
His -493	-	expression tag
His -492	-	expression tag
His -491	-	expression tag
His -490	-	expression tag
Ser -489	-	expression tag
Ser -488	-	expression tag
Gly -487	-	expression tag
Leu -486	-	expression tag
Glu -485	-	expression tag
Val -484	-	expression tag
Leu -483	-	expression tag
Phe -482	-	expression tag
Gln -481	-	expression tag
Gly -480	-	expression tag
Pro -479	-	expression tag
Gly -3	-	linker
Ser -2	-	linker
Gly -1	-	linker
Ser 0	-	linker
Gly 1	-	linker

R: 2 - 291 (UniProt: Q9NYV7)

PDB	External Database	Details
Cys 133	Ser 133	engineered mutation

R: 292 - 473 (PDB: 9K6L)

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	230673.0
Non-Polymers*	Number of molecules	1
	Total formula weight	286.3
All*	Total formula weight	230959.3

*Water molecules are not included.