9K4I

Cryo-EM structure of the human TRPC1/C5 heteromer

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C, D (A, C, D)	Maltose/maltodextrin-binding periplasmic protein,Short transient receptor potential channel 5	polymer	1203	136984.2	3	UniProt (P0AEX9) UniProt (Q9QX29) Pfam (PF12796) Pfam (PF00023) Pfam (PF08344) Pfam (PF00520)	Escherichia coli (strain K12)	MMBP,Maltodextrin-binding protein,Maltose-binding protein,MBP,TrpC5,Capacitative calcium entry channel 2,CCE2,Transient receptor protein 5,TRP-5,mTRP5,Trp-related protein 5
2	B (B)	Maltose/maltodextrin-binding periplasmic protein,Short transient receptor potential channel 1,Extended tegument protein pp150	polymer	1504	168800.6	1	UniProt (P0AEX9) UniProt (P48995) UniProt (A0A076JQ90) Pfam (PF08344) Pfam (PF00520)	Escherichia coli K-12	MMBP,Maltodextrin-binding protein,Maltose-binding protein,MBP,TrpC1,Transient receptor protein 1,TRP-1
3	E, I, M (A, C, D)	CALCIUM ION	non-polymer		40.1	3	Chemie (CA)
4	F, J, N (A, C, D)	CHOLESTEROL HEMISUCCINATE	non-polymer		486.7	3	Chemie (Y01)
5	G, H, K, L (A, B, C)	(2S)-2-(hexadecanoyloxy)-3-hydroxypropyl (9Z)-octadec-9-enoate	non-polymer		594.9	4	Chemie (YZY)

Sequence modifications

A, C, D: -391 - -26 (UniProt: P0AEX9)

PDB	External Database	Details
Met -437	-	initiating methionine
Gly -436	-	expression tag
Ser -435	-	expression tag
Ala -434	-	expression tag
Gly -433	-	expression tag
Asp -432	-	expression tag
Tyr -431	-	expression tag
Lys -430	-	expression tag
Asp -429	-	expression tag
His -428	-	expression tag
Asp -427	-	expression tag
Gly -426	-	expression tag
Asp -425	-	expression tag
Tyr -424	-	expression tag
Lys -423	-	expression tag
Asp -422	-	expression tag
His -421	-	expression tag
Asp -420	-	expression tag
Ile -419	-	expression tag
Asp -418	-	expression tag
Tyr -417	-	expression tag
Lys -416	-	expression tag
Asp -415	-	expression tag
Asp -414	-	expression tag
Asp -413	-	expression tag
Asp -412	-	expression tag
Lys -411	-	expression tag
Gly -410	-	expression tag
Ser -409	-	expression tag
Ala -408	-	expression tag
Ala -407	-	expression tag
Ala -406	-	expression tag
Met -405	-	expression tag
Gly -404	-	expression tag
Ser -403	-	expression tag
Ser -402	-	expression tag
His -401	-	expression tag
His -400	-	expression tag
His -399	-	expression tag
His -398	-	expression tag
His -397	-	expression tag
His -396	-	expression tag
Gly -395	-	expression tag
Ser -394	-	expression tag
Ser -393	-	expression tag
Met -392	-	expression tag
Asn -25	-	linker
Ser -24	-	linker
Ser -23	-	linker
Ser -22	-	linker
Asn -21	-	linker
Asn -20	-	linker
Asn -19	-	linker
Asn -18	-	linker
Asn -17	-	linker
Asn -16	-	linker
Asn -15	-	linker
Asn -14	-	linker
Asn -13	-	linker
Asn -12	-	linker
Leu -11	-	linker
Gly -10	-	linker
Ile -9	-	linker
Glu -8	-	linker
Leu -7	-	linker
Glu -6	-	linker
Val -5	-	linker
Leu -4	-	linker
Phe -3	-	linker
Gln -2	-	linker
Gly -1	-	linker
Pro 0	-	linker

A, C, D: 1 - 765 (UniProt: Q9QX29)
B: -391 - -26 (UniProt: P0AEX9)

PDB	External Database	Details
Ala -409	-	expression tag
Thr -408	-	expression tag
Met -407	-	expression tag
Gly -406	-	expression tag
Met -405	-	expression tag
Gly -404	-	expression tag
Ser -403	-	expression tag
Ser -402	-	expression tag
His -401	-	expression tag
His -400	-	expression tag
His -399	-	expression tag
His -398	-	expression tag
His -397	-	expression tag
His -396	-	expression tag
Gly -395	-	expression tag
Ser -394	-	expression tag
Ser -393	-	expression tag
Met -392	-	expression tag
Asn -25	-	linker
Ser -24	-	linker
Ser -23	-	linker
Ser -22	-	linker
Asn -21	-	linker
Asn -20	-	linker
Asn -19	-	linker
Asn -18	-	linker
Asn -17	-	linker
Asn -16	-	linker
Asn -15	-	linker
Asn -14	-	linker
Asn -13	-	linker
Asn -12	-	linker
Leu -11	-	linker
Gly -10	-	linker
Ile -9	-	linker
Glu -8	-	linker
Leu -7	-	linker
Glu -6	-	linker
Val -5	-	linker
Leu -4	-	linker
Phe -3	-	linker
Gln -2	-	linker
Gly -1	-	linker
Pro 0	-	linker

B: 1 - 793 (UniProt: P48995)

PDB	External Database	Details
Gly 794	-	linker
Gly 795	-	linker
Gly 796	-	linker
Ser 797	-	linker
Gly 798	-	linker
Gly 799	-	linker
Gly 800	-	linker
Ser 801	-	linker
Gly 802	-	linker
Gly 803	-	linker
Gly 804	-	linker
Ser 805	-	linker
Glu 806	-	linker
Asn 807	-	linker
Leu 808	-	linker
Tyr 809	-	linker
Phe 810	-	linker
Gln 811	-	linker
Gly 812	-	linker

B: 813 - 1057 (UniProt: A0A076JQ90)

PDB	External Database	Details
Gly 1058	-	expression tag
Ser 1059	-	expression tag
Ala 1060	-	expression tag
Gly 1061	-	expression tag
Ser 1062	-	expression tag
Ala 1063	-	expression tag
Gly 1064	-	expression tag
Ser 1065	-	expression tag
Ala 1066	-	expression tag
Trp 1067	-	expression tag
Ser 1068	-	expression tag
His 1069	-	expression tag
Pro 1070	-	expression tag
Gln 1071	-	expression tag
Phe 1072	-	expression tag
Glu 1073	-	expression tag
Lys 1074	-	expression tag
Gly 1075	-	expression tag
Gly 1076	-	expression tag
Gly 1077	-	expression tag
Ser 1078	-	expression tag
Gly 1079	-	expression tag
Gly 1080	-	expression tag
Gly 1081	-	expression tag
Ser 1082	-	expression tag
Gly 1083	-	expression tag
Gly 1084	-	expression tag
Gly 1085	-	expression tag
Ser 1086	-	expression tag
Trp 1087	-	expression tag
Ser 1088	-	expression tag
His 1089	-	expression tag
Pro 1090	-	expression tag
Gln 1091	-	expression tag
Phe 1092	-	expression tag
Glu 1093	-	expression tag
Lys 1094	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	579753.3
Non-Polymers*	Number of molecules	10
	Total formula weight	3960.2
All*	Total formula weight	583713.5

*Water molecules are not included.