9K4C
SuperFi Cas9 - 22nt sgRNA - DNA ternary complex
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | CRISPR-associated endonuclease Cas9/Csn1 | polymer | 1368 | 158502.4 | 1 | UniProt (Q99ZW2) | Streptococcus pyogenes | SpCas9,SpyCas9 |
| 2 | B (B) | DNA (50-MER) | polymer | 50 | 15438.9 | 1 | synthetic construct | ||
| 3 | C (C) | DNA (50-MER) | polymer | 50 | 15371.8 | 1 | synthetic construct | ||
| 4 | D (D) | RNA (102-MER) | polymer | 102 | 32815.5 | 1 | synthetic construct |
Sequence modifications
A: 1 - 1368 (UniProt: Q99ZW2)
| PDB | External Database | Details |
|---|---|---|
| Asp 1010 | Tyr 1010 | engineered mutation |
| Asp 1013 | Tyr 1013 | engineered mutation |
| Asp 1016 | Tyr 1016 | engineered mutation |
| Asp 1018 | Val 1018 | engineered mutation |
| Asp 1019 | Arg 1019 | engineered mutation |
| Asp 1027 | Gln 1027 | engineered mutation |
| Asp 1031 | Lys 1031 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 4 |
| Total formula weight | 222128.5 | |
| All* | Total formula weight | 222128.5 |
