9K3K
Cryo-EM structure of the unliganded human melanocortin receptor 4 (MC4R)-Gs complex
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (R) | Melanocortin receptor 4,Melanocortin receptor 4,Melanocortin receptor 4,LgBiT subunit | polymer | 505 | 55571.5 | 1 | UniProt (P32245) Pfam (PF00001) | Homo sapiens (human) | MC4-R |
| 2 | B (A) | Guanine nucleotide-binding protein G(i) subunit alpha-1,Guanine nucleotide-binding protein G(s) subunit alpha isoforms short | polymer | 361 | 41879.5 | 1 | UniProt (P63096) UniProt (P63092) Pfam (PF00503) | Homo sapiens (human) | Adenylate cyclase-inhibiting G alpha protein,Adenylate cyclase-stimulating G alpha protein |
| 3 | C (B) | Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1,Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1,Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1,HiBiT | polymer | 371 | 40227.0 | 1 | UniProt (P62873) Pfam (PF00400) | Homo sapiens (human) | Transducin beta chain 1 |
| 4 | D (G) | Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 | polymer | 71 | 7861.1 | 1 | UniProt (P63212) Pfam (PF00631) | Bos taurus (domestic cattle) | G gamma-I |
| 5 | E (N) | Nb35 | polymer | 160 | 17352.5 | 1 | synthetic construct | ||
| 6 | F (S) | scFv16 | polymer | 285 | 30363.0 | 1 | synthetic construct |
Sequence modifications
R: 1 - 332 (UniProt: P32245)
R: 348 - 505 (PDB: 9K3K)
A: 8 - 26 (UniProt: P63096)
A: 27 - 83 (UniProt: P63092)
A: 84 - 204 (UniProt: P63096)
A: 205 - 253 (UniProt: P63092)
A: 264 - 394 (UniProt: P63092)
B: 2 - 340 (UniProt: P62873)
B: 356 - 366 (PDB: 9K3K)
| PDB | External Database | Details |
|---|---|---|
| Gly 333 | - | linker |
| Ser 334 | - | linker |
| Ser 335 | - | linker |
| Gly 336 | - | linker |
| Gly 337 | - | linker |
| Gly 338 | - | linker |
| Gly 339 | - | linker |
| Ser 340 | - | linker |
| Gly 341 | - | linker |
| Gly 342 | - | linker |
| Gly 343 | - | linker |
| Gly 344 | - | linker |
| Ser 345 | - | linker |
| Ser 346 | - | linker |
| Gly 347 | - | linker |
A: 8 - 26 (UniProt: P63096)
A: 27 - 83 (UniProt: P63092)
| PDB | External Database | Details |
|---|---|---|
| Asp 49 | Gly 49 | engineered mutation |
| Asn 50 | Glu 50 | engineered mutation |
| Tyr 63 | Leu 63 | engineered mutation |
A: 205 - 253 (UniProt: P63092)
| PDB | External Database | Details |
|---|---|---|
| Ala 226 | Gly 226 | engineered mutation |
| Asp 249 | Ala 249 | engineered mutation |
| Asp 252 | Ser 252 | engineered mutation |
| PDB | External Database | Details |
|---|---|---|
| Asp 272 | Leu 272 | engineered mutation |
| Ser 366 | Ala 366 | engineered mutation |
| Ala 372 | Ile 372 | engineered mutation |
| Ile 375 | Val 375 | engineered mutation |
| PDB | External Database | Details |
|---|---|---|
| Met -4 | - | initiating methionine |
| Gly -3 | - | expression tag |
| Ser -2 | - | expression tag |
| Leu -1 | - | expression tag |
| Leu 0 | - | expression tag |
| Gln 1 | - | expression tag |
| Gly 341 | - | linker |
| Ser 342 | - | linker |
| Ser 343 | - | linker |
| Gly 344 | - | linker |
| Gly 345 | - | linker |
| Gly 346 | - | linker |
| Gly 347 | - | linker |
| Ser 348 | - | linker |
| Gly 349 | - | linker |
| Gly 350 | - | linker |
| Gly 351 | - | linker |
| Gly 352 | - | linker |
| Ser 353 | - | linker |
| Ser 354 | - | linker |
| Gly 355 | - | linker |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 6 |
| Total formula weight | 193254.6 | |
| All* | Total formula weight | 193254.6 |
