9K32
Cryo-EM structure of the NbaCas9-guide RNA-target DNA complex
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | HNH nuclease domain-containing protein | polymer | 736 | 84610.7 | 1 | UniProt (A0A3C0V2Q6) | Nitrospirota bacterium | |
| 2 | B (B) | Guide RNA | polymer | 195 | 62928.3 | 1 | Nitrospirota bacterium | ||
| 3 | C (C) | Target DNA strand | polymer | 43 | 12973.3 | 1 | synthetic construct | ||
| 4 | D (D) | Non-target DNA strand | polymer | 43 | 13498.7 | 1 | synthetic construct | ||
| 5 | E, H, I (A, B) | MAGNESIUM ION | non-polymer | 24.3 | 3 | Chemie (MG) | |||
| 6 | F, G (A) | ZINC ION | non-polymer | 65.4 | 2 | Chemie (ZN) | |||
| 7 | J (B) | water | water | 18.0 | 9 | Chemie (HOH) |
Sequence modifications
A: 2 - 735 (UniProt: A0A3C0V2Q6)
| PDB | External Database | Details |
|---|---|---|
| Gly 0 | - | expression tag |
| Ser 1 | - | expression tag |
| Ala 10 | Asp 10 | conflict |
| Ala 364 | His 364 | conflict |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 4 |
| Total formula weight | 174011.0 | |
| Non-Polymers* | Number of molecules | 5 |
| Total formula weight | 203.7 | |
| All* | Total formula weight | 174214.7 |
