9K27

PrRP31 bound prolactin-releasing peptide receptor coupled with Gq protein complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Prolactin-releasing peptide receptor	polymer	370	41163.5	1	UniProt (P49683) Pfam (PF00001)	Homo sapiens (human)	PrRP receptor,PrRPR,G-protein coupled receptor 10,hGR3
2	B (B)	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	357	39286.9	1	UniProt (P62873) Pfam (PF00400)	Homo sapiens (human)	Transducin beta chain 1
3	C (C)	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	polymer	71	7851.1	1	UniProt (P59768) Pfam (PF00631)	Homo sapiens (human)	G gamma-I
4	D (D)	scfv16	polymer	261	27707.9	1		Homo sapiens
5	E (E)	Guanine nucleotide-binding protein G(q) subunit alpha	polymer	353	41381.1	1	UniProt (P50148) Pfam (PF00503)	Homo sapiens (human)	Guanine nucleotide-binding protein alpha-q
6	F (F)	Prolactin-releasing peptide PrRP31	polymer	32	3671.2	1	UniProt (P81277) Pfam (PF15172)	Homo sapiens

Sequence modifications

B: 2 - 340 (UniProt: P62873)

PDB	External Database	Details
His -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
Leu -10	-	expression tag
Glu -9	-	expression tag
Val -8	-	expression tag
Leu -7	-	expression tag
Phe -6	-	expression tag
Gln -5	-	expression tag
Gly -4	-	expression tag
Pro -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
Ser 0	-	expression tag
Gly 1	-	expression tag

C: 1 - 71 (UniProt: P59768)

PDB	External Database	Details
Phe 62	Arg 62	conflict

E: 19 - 353 (UniProt: P50148)

PDB	External Database	Details
Met 1	-	initiating methionine
Gly 2	-	expression tag
Cys 3	-	expression tag
Thr 4	-	expression tag
Leu 5	-	expression tag
Ser 6	-	expression tag
Ala 7	-	expression tag
Glu 8	-	expression tag
Asp 9	-	expression tag
Lys 10	-	expression tag
Ala 11	-	expression tag
Ala 12	-	expression tag
Val 13	-	expression tag
Glu 14	-	expression tag
Arg 15	-	expression tag
Ser 16	-	expression tag
Lys 17	-	expression tag
Met 18	-	expression tag

F: 1 - 31 (UniProt: P81277)

PDB	External Database	Details
Nh2 33	-	amidation

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	161061.7
All*	Total formula weight	161061.7

*Water molecules are not included.