9K26

PrRP31 bound prolactin-releasing peptide receptor coupled with Gi protein complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Prolactin-releasing peptide receptor	polymer	370	41163.5	1	UniProt (P49683) Pfam (PF00001)	Homo sapiens (human)	PrRP receptor,PrRPR,G-protein coupled receptor 10,hGR3
2	B (F)	Prolactin-releasing peptide PrRP31	polymer	32	3671.2	1	UniProt (P81277) Pfam (PF15172)	Homo sapiens
3	C (C)	Guanine nucleotide-binding protein G(i) subunit alpha-1	polymer	351	40153.7	1	UniProt (P63096) Pfam (PF00503)	Homo sapiens (human)	Adenylate cyclase-inhibiting G alpha protein
4	D (B)	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	357	39286.9	1	UniProt (P62873) Pfam (PF00400)	Homo sapiens (human)	Transducin beta chain 1
5	E (G)	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	polymer	71	7861.1	1	UniProt (P59768) Pfam (PF00631)	Homo sapiens (human)	G gamma-I
6	F (S)	scfv16	polymer	261	27707.9	1		Homo sapiens

Sequence modifications

F: 1 - 31 (UniProt: P81277)

PDB	External Database	Details
Nh2 33	-	amidation

C: 4 - 354 (UniProt: P63096)

PDB	External Database	Details
Ala 203	Gly 203	engineered mutation
Ser 326	Ala 326	engineered mutation

B: 2 - 340 (UniProt: P62873)

PDB	External Database	Details
His -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
Leu -10	-	expression tag
Glu -9	-	expression tag
Val -8	-	expression tag
Leu -7	-	expression tag
Phe -6	-	expression tag
Gln -5	-	expression tag
Gly -4	-	expression tag
Pro -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
Ser 0	-	expression tag
Gly 1	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	159844.3
All*	Total formula weight	159844.3

*Water molecules are not included.