9K25

Cryo-EM structure of apo-P2Y purinoceptor 2-miniGq-Nb35 complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Guanine nucleotide-binding protein G(s) subunit alpha isoforms short	polymer	243	28014.8	1	UniProt (by SIFTS) (A0A804HIH4)	Homo sapiens (human)
2	B (B)	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	358	39418.1	1	UniProt (P62873) Pfam (PF25391)	Homo sapiens (human)
3	C (G)	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	polymer	71	7861.1	1	UniProt (P59768) Pfam (PF00631)	Homo sapiens (human)
4	D (I)	P2Y purinoceptor 2	polymer	420	47053.7	1	UniProt (P41231) Pfam (PF00001)	Homo sapiens (human)	P2Y2,ATP receptor,P2U purinoceptor 1,P2U1,P2U receptor 1,Purinergic receptor
5	E (N)	Nanobody 35	polymer	134	14686.3	1		Vicugna pacos

Sequence modifications

A: 11 - 203 (PDB: 9K25)
A: 204 - 394 (PDB: 9K25)
B: 2 - 340 (UniProt: P62873)

PDB	External Database	Details
Met -17	-	initiating methionine
His -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
Leu -10	-	expression tag
Glu -9	-	expression tag
Val -8	-	expression tag
Leu -7	-	expression tag
Phe -6	-	expression tag
Gln -5	-	expression tag
Gly -4	-	expression tag
Pro -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
Ser 0	-	expression tag
Gly 1	-	expression tag

I: 1 - 377 (UniProt: P41231)

PDB	External Database	Details
Met -23	-	initiating methionine
Lys -22	-	expression tag
Thr -21	-	expression tag
Ile -20	-	expression tag
Ile -19	-	expression tag
Ala -18	-	expression tag
Leu -17	-	expression tag
Ser -16	-	expression tag
Tyr -15	-	expression tag
Ile -14	-	expression tag
Phe -13	-	expression tag
Cys -12	-	expression tag
Leu -11	-	expression tag
Val -10	-	expression tag
Phe -9	-	expression tag
Ala -8	-	expression tag
Asp -7	-	expression tag
Tyr -6	-	expression tag
Lys -5	-	expression tag
Asp -4	-	expression tag
Asp -3	-	expression tag
Asp -2	-	expression tag
Asp -1	-	expression tag
Ala 0	-	expression tag
Leu 46	Pro 46	variant
Asn 302	Asp 302	engineered mutation
Ser 312	Arg 312	variant
His 378	-	expression tag
His 379	-	expression tag
His 380	-	expression tag
His 381	-	expression tag
His 382	-	expression tag
His 383	-	expression tag
Gly 384	-	expression tag
Gly 385	-	expression tag
Ser 386	-	expression tag
Gly 387	-	expression tag
Gly 388	-	expression tag
Leu 389	-	expression tag
Glu 390	-	expression tag
Val 391	-	expression tag
Leu 392	-	expression tag
Phe 393	-	expression tag
Gln 394	-	expression tag
Gly 395	-	expression tag
Pro 396	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	137034.0
All*	Total formula weight	137034.0

*Water molecules are not included.