9K1D

Cryo-EM structure of the butyrate bound FFA2-Gi complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (R)	Free fatty acid receptor 2	polymer	384	42959.0	1	UniProt (O15552) Pfam (PF00001)	Homo sapiens (human)	G-protein coupled receptor 43
2	B (A)	Guanine nucleotide-binding protein G(i) subunit alpha-1	polymer	354	40447.1	1	UniProt (P63096) Pfam (PF00503)	Homo sapiens (human)	Adenylate cyclase-inhibiting G alpha protein
3	C (B)	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	340	37416.9	1	UniProt (P62873) Pfam (PF25391)	Homo sapiens (human)	Transducin beta chain 1
4	D (C)	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	polymer	71	7861.1	1	UniProt (P59768) Pfam (PF00631)	Homo sapiens (human)	G gamma-I
5	E (R)	butanoic acid	non-polymer		88.1	1	Chemie (BUA)
6	F, G, H, I, J (R)	CHOLESTEROL	non-polymer		386.7	5	Chemie (CLR)

Sequence modifications

R: 1 - 330 (UniProt: O15552)

PDB	External Database	Details
Met -53	-	initiating methionine
Lys -52	-	expression tag
Thr -51	-	expression tag
Ile -50	-	expression tag
Ile -49	-	expression tag
Ala -48	-	expression tag
Leu -47	-	expression tag
Ser -46	-	expression tag
Tyr -45	-	expression tag
Ile -44	-	expression tag
Phe -43	-	expression tag
Cys -42	-	expression tag
Leu -41	-	expression tag
Val -40	-	expression tag
Phe -39	-	expression tag
Ala -38	-	expression tag
Asp -37	-	expression tag
Tyr -36	-	expression tag
Lys -35	-	expression tag
Asp -34	-	expression tag
Asp -33	-	expression tag
Asp -32	-	expression tag
Asp -31	-	expression tag
Trp -30	-	expression tag
Ser -29	-	expression tag
His -28	-	expression tag
Pro -27	-	expression tag
Gln -26	-	expression tag
Phe -25	-	expression tag
Glu -24	-	expression tag
Lys -23	-	expression tag
Gly -22	-	expression tag
Gly -21	-	expression tag
Gly -20	-	expression tag
Ser -19	-	expression tag
Gly -18	-	expression tag
Gly -17	-	expression tag
Gly -16	-	expression tag
Ser -15	-	expression tag
Gly -14	-	expression tag
Gly -13	-	expression tag
Ser -12	-	expression tag
Ala -11	-	expression tag
Trp -10	-	expression tag
Ser -9	-	expression tag
His -8	-	expression tag
Pro -7	-	expression tag
Gln -6	-	expression tag
Phe -5	-	expression tag
Glu -4	-	expression tag
Lys -3	-	expression tag
Gly -2	-	expression tag
Ala -1	-	expression tag
Pro 0	-	expression tag

A: 1 - 354 (UniProt: P63096)

PDB	External Database	Details
Cys 47	Ser 47	engineered mutation
Thr 202	Gly 202	engineered mutation
Ala 203	Gly 203	engineered mutation
Ala 245	Glu 245	engineered mutation
Ser 326	Ala 326	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	128684.2
Non-Polymers*	Number of molecules	6
	Total formula weight	2021.4
All*	Total formula weight	130705.6

*Water molecules are not included.