9K1C
Cryo-EM structure of the DHA bound FFA1-Gi complex
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (R) | Free fatty acid receptor 1 | polymer | 357 | 37670.7 | 1 | UniProt (O14842) Pfam (PF00001) | Homo sapiens (human) | G-protein coupled receptor 40 |
| 2 | B (A) | Guanine nucleotide-binding protein G(i) subunit alpha-1 | polymer | 354 | 40447.1 | 1 | UniProt (P63096) Pfam (PF00503) | Homo sapiens (human) | Adenylate cyclase-inhibiting G alpha protein |
| 3 | C (B) | Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 | polymer | 340 | 37416.9 | 1 | UniProt (P62873) Pfam (PF00400) | Homo sapiens (human) | Transducin beta chain 1 |
| 4 | D (C) | Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 | polymer | 71 | 7861.1 | 1 | UniProt (P59768) Pfam (PF00631) | Homo sapiens (human) | G gamma-I |
| 5 | E (R) | DOCOSA-4,7,10,13,16,19-HEXAENOIC ACID | non-polymer | 328.5 | 1 | Chemie (HXA) |
Sequence modifications
R: 1 - 300 (UniProt: O14842)
A: 1 - 354 (UniProt: P63096)
| PDB | External Database | Details |
|---|---|---|
| Met -46 | - | initiating methionine |
| Lys -45 | - | expression tag |
| Thr -44 | - | expression tag |
| Ile -43 | - | expression tag |
| Ile -42 | - | expression tag |
| Ala -41 | - | expression tag |
| Leu -40 | - | expression tag |
| Ser -39 | - | expression tag |
| Tyr -38 | - | expression tag |
| Ile -37 | - | expression tag |
| Phe -36 | - | expression tag |
| Cys -35 | - | expression tag |
| Leu -34 | - | expression tag |
| Val -33 | - | expression tag |
| Phe -32 | - | expression tag |
| Ala -31 | - | expression tag |
| Trp -30 | - | expression tag |
| Ser -29 | - | expression tag |
| His -28 | - | expression tag |
| Pro -27 | - | expression tag |
| Gln -26 | - | expression tag |
| Phe -25 | - | expression tag |
| Glu -24 | - | expression tag |
| Lys -23 | - | expression tag |
| Gly -22 | - | expression tag |
| Gly -21 | - | expression tag |
| Gly -20 | - | expression tag |
| Ser -19 | - | expression tag |
| Gly -18 | - | expression tag |
| Gly -17 | - | expression tag |
| Gly -16 | - | expression tag |
| Ser -15 | - | expression tag |
| Gly -14 | - | expression tag |
| Gly -13 | - | expression tag |
| Ser -12 | - | expression tag |
| Ala -11 | - | expression tag |
| Trp -10 | - | expression tag |
| Ser -9 | - | expression tag |
| His -8 | - | expression tag |
| Pro -7 | - | expression tag |
| Gln -6 | - | expression tag |
| Phe -5 | - | expression tag |
| Glu -4 | - | expression tag |
| Lys -3 | - | expression tag |
| Gly -2 | - | expression tag |
| Ala -1 | - | expression tag |
| Pro 0 | - | expression tag |
| Glu 301 | - | expression tag |
| Phe 302 | - | expression tag |
| Asp 303 | - | expression tag |
| Tyr 304 | - | expression tag |
| Lys 305 | - | expression tag |
| Asp 306 | - | expression tag |
| Asp 307 | - | expression tag |
| Asp 308 | - | expression tag |
| Asp 309 | - | expression tag |
| Lys 310 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Cys 47 | Ser 47 | engineered mutation |
| Thr 202 | Gly 202 | engineered mutation |
| Ala 203 | Gly 203 | engineered mutation |
| Ala 245 | Glu 245 | engineered mutation |
| Ser 326 | Ala 326 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 4 |
| Total formula weight | 123395.9 | |
| Non-Polymers* | Number of molecules | 1 |
| Total formula weight | 328.5 | |
| All* | Total formula weight | 123724.4 |
