9K18
A cryo-EM structure of B. oleracea RNA polymerase V in complex with 7U sacffold at 3.42 Angstrom
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | DNA-directed RNA polymerase V largest subunit | polymer | 2032 | 222876.9 | 1 | Pfam (PF04997) Pfam (PF00623) Pfam (PF04983) Pfam (PF04998) Pfam (PF11523) UniProt (by SIFTS) (A0A0D3B5Z0) | Brassica oleracea (wild cabbage) | |
| 2 | B (B) | DNA-directed RNA polymerase subunit beta | polymer | 1169 | 132377.8 | 1 | Brassica oleracea (wild cabbage) | ||
| 3 | C (C) | DNA-directed RNA polymerase RpoA/D/Rpb3-type domain-containing protein | polymer | 319 | 35484.1 | 1 | UniProt (A0A0D3D418) Pfam (PF01193) Pfam (PF01000) | Brassica oleracea (wild cabbage) | DNA-directed RNA polymerase II, IV and V subunit 3 |
| 4 | D (E) | RNA polymerase subunit H/Rpb5 C-terminal domain-containing protein | polymer | 230 | 26260.1 | 1 | UniProt (A0A0D3DTU3) Pfam (PF03871) Pfam (PF01191) | Brassica oleracea (wild cabbage) | |
| 5 | E (F) | DNA-directed RNA polymerase RpoA/D/Rpb3-type domain-containing protein | polymer | 144 | 16686.3 | 1 | UniProt (A0A0D3BZZ8) Pfam (PF01192) | Brassica oleracea (wild cabbage) | DNA-directed RNA polymerase V subunit 6 |
| 6 | F (H) | DNA-directed RNA polymerase II, IV and V subunit 8 | polymer | 146 | 16607.3 | 1 | UniProt (A0A0D3DUD8) Pfam (PF03870) | Brassica oleracea (wild cabbage) | |
| 7 | G (I) | DNA-directed RNA polymerase subunit | polymer | 114 | 13123.8 | 1 | UniProt (A0A0D3A7P5) Pfam (PF02150) Pfam (PF01096) | Brassica oleracea (wild cabbage) | DNA-directed RNA polymerase V subunit 9 |
| 8 | H (J) | DNA-directed RNA polymerase II, IV and V subunit 10 | polymer | 71 | 8167.5 | 1 | UniProt (A0A0D3AAT3) Pfam (PF01194) | Brassica oleracea (wild cabbage) | |
| 9 | I (K) | DNA-directed RNA polymerase RBP11-like dimerisation domain-containing protein | polymer | 116 | 13538.3 | 1 | UniProt (A0A0D3DS91) Pfam (PF13656) | Brassica oleracea (wild cabbage) | |
| 10 | J (L) | DNA-directed RNA polymerase II, IV and V subunit 12 | polymer | 51 | 5983.8 | 1 | UniProt (A0A0D2ZPP3) Pfam (PF03604) | Brassica oleracea (wild cabbage) | |
| 11 | K (T) | DNA (34-MER) | polymer | 34 | 10484.8 | 1 | Brassica oleracea | ||
| 12 | L (N) | DNA (34-MER) | polymer | 34 | 10433.7 | 1 | Brassica oleracea | ||
| 13 | M, O, P, Q, R... (A, C, H, I, J...) | ZINC ION | non-polymer | 65.4 | 7 | Chemie (ZN) PubChem (32051) | |||
| 14 | N (A) | CALCIUM ION | non-polymer | 40.1 | 1 | Chemie (CA) PubChem (271) |
Sequence modifications
C: 1 - 319 (UniProt: A0A0D3D418)
E: 1 - 230 (UniProt: A0A0D3DTU3)
F: 1 - 144 (UniProt: A0A0D3BZZ8)
I: 1 - 114 (UniProt: A0A0D3A7P5)
L: 1 - 51 (UniProt: A0A0D2ZPP3)
| PDB | External Database | Details |
|---|---|---|
| Thr 3 | Ser 3 | conflict |
| PDB | External Database | Details |
|---|---|---|
| Gly 101 | Ser 101 | conflict |
| Gln 182 | His 182 | conflict |
| Ile 210 | Val 210 | conflict |
| PDB | External Database | Details |
|---|---|---|
| Glu 51 | Asp 51 | conflict |
| PDB | External Database | Details |
|---|---|---|
| Arg 20 | Lys 20 | conflict |
| Asp 40 | Asn 40 | conflict |
| PDB | External Database | Details |
|---|---|---|
| Glu 18 | Lys 18 | conflict |
| Cys 32 | Arg 32 | conflict |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 12 |
| Total formula weight | 512024.4 | |
| Non-Polymers* | Number of molecules | 8 |
| Total formula weight | 497.9 | |
| All* | Total formula weight | 512522.3 |
