9K0K

Cryo-EM structure of UTP-bound P2Y purinoceptor 4-miniGq-Nb35 complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Guanine nucleotide-binding protein G(s) subunit alpha isoforms short	polymer	243	28014.8	1	UniProt (P63092) Pfam (PF00503)	Homo sapiens (human)	Adenylate cyclase-stimulating G alpha protein
2	B (B)	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	358	39418.1	1	UniProt (P62873) Pfam (PF25391)	Homo sapiens (human)	Transducin beta chain 1
3	C (G)	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	polymer	71	7861.1	1	UniProt (P59768) Pfam (PF00631)	Homo sapiens (human)	G gamma-I
4	D (N)	Nanobody 35	polymer	134	14686.3	1		Vicugna pacos
5	E (I)	P2Y purinoceptor 4	polymer	408	45787.2	1	UniProt (P51582) Pfam (PF00001)	Homo sapiens (human)	P2Y4,P2P,Uridine nucleotide receptor,UNR
6	F (I)	URIDINE 5'-TRIPHOSPHATE	non-polymer		484.1	1	Chemie (UTP)

Sequence modifications

A: 26 - 195 (UniProt: P63092)

PDB	External Database	Details
Thr 11	-	expression tag
Leu 12	-	expression tag
Ser 13	-	expression tag
Ala 14	-	expression tag
Glu 15	-	expression tag
Asp 16	-	expression tag
Lys 17	-	expression tag
Ala 18	-	expression tag
Ala 19	-	expression tag
Val 20	-	expression tag
Glu 21	-	expression tag
Arg 22	-	expression tag
Ser 23	-	expression tag
Lys 24	-	expression tag
Met 25	-	expression tag
Asp 49	Gly 49	conflict
Asn 50	Glu 50	conflict

A: 204 - 394 (UniProt: P63092)

PDB	External Database	Details
Gly 196	-	linker
Gly 197	-	linker
Ser 198	-	linker
Gly 199	-	linker
Gly 200	-	linker
Ser 201	-	linker
Gly 202	-	linker
Gly 203	-	linker
Asp 249	Ala 249	conflict
Asp 252	Ser 252	conflict
-	Asn 254	deletion
-	Met 255	deletion
-	Val 256	deletion
-	Ile 257	deletion
-	Arg 258	deletion
-	Glu 259	deletion
-	Asp 260	deletion
-	Asn 261	deletion
-	Gln 262	deletion
-	Thr 263	deletion
Asp 272	Leu 272	conflict
Ala 372	Ile 372	conflict
Ile 375	Val 375	conflict
Lys 380	Arg 380	conflict
Leu 384	Gln 384	conflict
Gln 385	Arg 385	conflict
Asn 387	His 387	conflict
Glu 390	Gln 390	conflict
Asn 392	Glu 392	conflict
Val 394	Leu 394	conflict

B: 2 - 340 (UniProt: P62873)

PDB	External Database	Details
Met -17	-	initiating methionine
His -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
Leu -10	-	expression tag
Glu -9	-	expression tag
Val -8	-	expression tag
Leu -7	-	expression tag
Phe -6	-	expression tag
Gln -5	-	expression tag
Gly -4	-	expression tag
Pro -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
Ser 0	-	expression tag
Gly 1	-	expression tag

I: 1 - 365 (UniProt: P51582)

PDB	External Database	Details
Met -23	-	initiating methionine
Lys -22	-	expression tag
Thr -21	-	expression tag
Ile -20	-	expression tag
Ile -19	-	expression tag
Ala -18	-	expression tag
Leu -17	-	expression tag
Ser -16	-	expression tag
Tyr -15	-	expression tag
Ile -14	-	expression tag
Phe -13	-	expression tag
Cys -12	-	expression tag
Leu -11	-	expression tag
Val -10	-	expression tag
Phe -9	-	expression tag
Ala -8	-	expression tag
Asp -7	-	expression tag
Tyr -6	-	expression tag
Lys -5	-	expression tag
Asp -4	-	expression tag
Asp -3	-	expression tag
Asp -2	-	expression tag
Asp -1	-	expression tag
Ala 0	-	expression tag
His 366	-	expression tag
His 367	-	expression tag
His 368	-	expression tag
His 369	-	expression tag
His 370	-	expression tag
His 371	-	expression tag
Gly 372	-	expression tag
Gly 373	-	expression tag
Ser 374	-	expression tag
Gly 375	-	expression tag
Gly 376	-	expression tag
Leu 377	-	expression tag
Glu 378	-	expression tag
Val 379	-	expression tag
Leu 380	-	expression tag
Phe 381	-	expression tag
Gln 382	-	expression tag
Gly 383	-	expression tag
Pro 384	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	135767.5
Non-Polymers*	Number of molecules	1
	Total formula weight	484.1
All*	Total formula weight	136251.7

*Water molecules are not included.