9JXS
Cryo-EM structure of Cas5-HNH Cascade bound with dsDNA
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (C) | CRISPR-associated protein Cse1 (CRISPR_cse1) | polymer | 535 | 60665.4 | 1 | UniProt (A0A1V6F8D1) UniProt (by SIFTS) (A0ACD6BA32) | Candidatus Cloacimonetes bacterium ADurb.Bin088 | |
| 2 | B (E) | CRISPR-associated protein Cse2 (CRISPR_cse2) | polymer | 174 | 20300.6 | 1 | UniProt (A0A1V6F8C9) UniProt (by SIFTS) (A0ACD6BA33) | Candidatus Cloacimonetes bacterium ADurb.Bin088 | Cas11 |
| 3 | C, D, E, F, G... (F, H, I, J, K...) | CRISPR system Cascade subunit CasC | polymer | 378 | 41794.4 | 6 | UniProt (A0A1V6F8B5) UniProt (by SIFTS) (A0ACD6BA34) | Candidatus Cloacimonetes bacterium ADurb.Bin088 | Cas7 |
| 4 | I (B) | CRISPR system Cascade subunit CasD | polymer | 388 | 43554.3 | 1 | UniProt (A0A1V6F8C5) UniProt (by SIFTS) (A0ACD6BA35) | Candidatus Cloacimonetes bacterium ADurb.Bin088 | |
| 5 | J (M) | DNA (54-MER) | polymer | 54 | 16524.6 | 1 | Escherichia coli | ||
| 6 | K (D) | CRISPR-associated endoribonuclease Cse3 | polymer | 272 | 31218.8 | 1 | UniProt (A0A1V6F8C4) UniProt (by SIFTS) (A0ACD6BA36) | Candidatus Cloacimonetes bacterium ADurb.Bin088 | Cas6 |
| 7 | L (A) | RNA (61-MER) | polymer | 61 | 19697.7 | 1 | Escherichia coli | ||
| 8 | M (N) | non-target DNA | polymer | 54 | 16751.8 | 1 | Escherichia coli | ||
| 9 | N, O (C, B) | MAGNESIUM ION | non-polymer | 24.3 | 2 | Chemie (MG) |
Sequence modifications
B: 1 - 388 (UniProt: A0A1V6F8C5)
| PDB | External Database | Details |
|---|---|---|
| Ala 333 | His 333 | conflict |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 13 |
| Total formula weight | 459479.3 | |
| Non-Polymers* | Number of molecules | 2 |
| Total formula weight | 48.6 | |
| All* | Total formula weight | 459527.9 |
