9JWK
Cryo-EM structure of FrCas9 in complex with sgRNA and 26-nt TS and 4-nt NTS substrates
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | CRISPR-associated endonuclease Cas9 | polymer | 1372 | 159661.7 | 1 | UniProt (A0A1Q9YNK3) Pfam (PF16592) Pfam (PF13395) Pfam (PF22702) Pfam (PF16595) | Faecalibaculum rodentium | FrCas9 |
| 2 | B (B) | sgRNA | polymer | 125 | 40071.6 | 1 | Homo sapiens | RNA (125-mer) | |
| 3 | C (D) | DNA (26-mer) | polymer | 26 | 7943.1 | 1 | Homo sapiens | ||
| 4 | D (D) | water | water | 18.0 | 1 | Chemie (HOH) |
Sequence modifications
A: 1 - 1372 (UniProt: A0A1Q9YNK3)
| PDB | External Database | Details |
|---|---|---|
| Ala 877 | His 877 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 3 |
| Total formula weight | 207676.4 | |
| All* | Total formula weight | 207676.4 |
