9JVJ

Structure of P450BytO mutant V219H in complex with pentapeptide MRYYH

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Putative cytochrome P450	polymer	398	43561.5	1	UniProt (A0A891LWT6)	Planomonospora sp.
2	B (B)	MET-ARG-TYR-TYR-HIS	polymer	5	770.9	1		synthetic construct
3	C (A)	PROTOPORPHYRIN IX CONTAINING FE	non-polymer		616.5	1	Chemie (HEM)
4	D, E (A, B)	water	water		18.0	369	Chemie (HOH)

Sequence modifications

A: 1 - 378 (UniProt: A0A891LWT6)

PDB	External Database	Details
Met -19	-	initiating methionine
Gly -18	-	expression tag
Ser -17	-	expression tag
Ser -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
Ser -9	-	expression tag
Ser -8	-	expression tag
Gly -7	-	expression tag
Leu -6	-	expression tag
Val -5	-	expression tag
Pro -4	-	expression tag
Arg -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
His 0	-	expression tag
His 219	Val 219	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	44332.4
Non-Polymers*	Number of molecules	1
	Total formula weight	616.5
All*	Total formula weight	44948.8

*Water molecules are not included.