9JP3

COMPLEX STRUCTURE OF ENDO-1,3-FUCANASE MUTANT D171A WITH FUCOTETRAOSE

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Glycoside-hydrolase family GH114 TIM-barrel domain-containing protein	polymer	409	46722.7	1	UniProt (A0A5C6D8T6) Pfam (PF14885)	Novipirellula artificiosorum
2	B (B)	2-O-sulfo-alpha-L-fucopyranose-(1-3)-alpha-L-fucopyranose-(1-3)-2,4-di-O-sulfo-alpha-L-fucopyranose-(1-3)-2-O-sulfo-alpha-L-fucopyranose	branched		922.8	1
3	C (A)	water	water		18.0	538	Chemie (HOH)

Sequence modifications

A: 1 - 373 (UniProt: A0A5C6D8T6)

PDB	External Database	Details
Met -35	-	initiating methionine
Gly -34	-	expression tag
Ser -33	-	expression tag
Ser -32	-	expression tag
His -31	-	expression tag
His -30	-	expression tag
His -29	-	expression tag
His -28	-	expression tag
His -27	-	expression tag
His -26	-	expression tag
Ser -25	-	expression tag
Ser -24	-	expression tag
Gly -23	-	expression tag
Leu -22	-	expression tag
Val -21	-	expression tag
Pro -20	-	expression tag
Arg -19	-	expression tag
Gly -18	-	expression tag
Ser -17	-	expression tag
His -16	-	expression tag
Met -15	-	expression tag
Ala -14	-	expression tag
Ser -13	-	expression tag
Met -12	-	expression tag
Thr -11	-	expression tag
Gly -10	-	expression tag
Gly -9	-	expression tag
Gln -8	-	expression tag
Gln -7	-	expression tag
Met -6	-	expression tag
Gly -5	-	expression tag
Arg -4	-	expression tag
Gly -3	-	expression tag
Ser -2	-	expression tag
Glu -1	-	expression tag
Phe 0	-	expression tag
Ala 171	Asp 193	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	46722.7
Branched	Number of molecules	1
	Total formula weight	922.8
All*	Total formula weight	47645.6

*Water molecules are not included.