9JOU

Cryo-EM structure of the zeaxanthin-bound light-driven proton pumping rhodopsin, NM-R1

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D, E (A, B, C, D, E)	Proteorhodopsin	polymer	260	29481.5	5	UniProt (W8VZ92) Pfam (PF01036)	Nonlabens marinus S1-08	NM-R1
2	BA, F, IA, N, U (D, A, E, B, C)	RETINAL	non-polymer		284.4	5	Chemie (RET)
3	AA, CA, DA, EA, G... (D, A, E, B, C)	HEXADECANE	non-polymer		226.4	13	Chemie (R16)
4	FA, HA, I, J, L... (D, E, A, B, C)	DODECANE	non-polymer		170.3	12	Chemie (D12)
5	GA, K, NA, R, Z (D, A, E, B, C)	Zeaxanthin	non-polymer		568.9	5	Chemie (K3I)
6	OA, PA, QA, RA, SA (A, B, C, D, E)	water	water		18.0	51	Chemie (HOH)

Sequence modifications

A, B, C, D, E: 1 - 246 (UniProt: W8VZ92)

PDB	External Database	Details
Leu 247	-	expression tag
Glu 248	-	expression tag
Glu 249	-	expression tag
Asn 250	-	expression tag
Leu 251	-	expression tag
Tyr 252	-	expression tag
Phe 253	-	expression tag
Gln 254	-	expression tag
Gly 255	-	expression tag
Trp 256	-	expression tag
Ser 257	-	expression tag
His 258	-	expression tag
Pro 259	-	expression tag
Gln 260	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	147407.3
Non-Polymers*	Number of molecules	35
	Total formula weight	9254.3
All*	Total formula weight	156661.6

*Water molecules are not included.