9JMJ

Cryo-EM structure of the GD-BatCoV (BtCoV/Ii/GD/2014-422) RBD in complex with human DPP4

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, C)	Dipeptidyl peptidase 4 soluble form,Isoform 1 of Immunoglobulin heavy constant gamma 1	polymer	998	114306.7	2	UniProt (P27487) UniProt (P01857) Pfam (PF00930) Pfam (PF00326) Pfam (PF07654)	Homo sapiens (human)	Dipeptidyl peptidase IV soluble form,Ig gamma-1 chain C region,Ig gamma-1 chain C region EU,Ig gamma-1 chain C region KOL,Ig gamma-1 chain C region NIE
2	C (B)	Spike glycoprotein,Isoform 1 of Immunoglobulin heavy constant gamma 1	polymer	502	55544.8	1	UniProt (A0A2R4KP93) UniProt (P01857) Pfam (PF09408) Pfam (PF07654)	Middle East respiratory syndrome-related coronavirus	GD-BatCoV,S glycoprotein,E2,Peplomer protein,Ig gamma-1 chain C region,Ig gamma-1 chain C region EU,Ig gamma-1 chain C region KOL,Ig gamma-1 chain C region NIE
3	D, F, G, H, I... (D, F, G, H, I...)	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		424.4	7	In PDB GlyTouCan (G42666HT)
4	E (E)	beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		586.5	1	In PDB GlyTouCan (G15407YE)
5	L, M, N, O, P... (A, C)	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	8	Chemie (NAG)

Sequence modifications

A, C: 39 - 766 (UniProt: P27487)

PDB	External Database	Details
Met 15	-	initiating methionine
Pro 16	-	expression tag
Met 17	-	expression tag
Gly 18	-	expression tag
Ser 19	-	expression tag
Leu 20	-	expression tag
Gln 21	-	expression tag
Pro 22	-	expression tag
Leu 23	-	expression tag
Ala 24	-	expression tag
Thr 25	-	expression tag
Leu 26	-	expression tag
Tyr 27	-	expression tag
Leu 28	-	expression tag
Leu 29	-	expression tag
Gly 30	-	expression tag
Met 31	-	expression tag
Leu 32	-	expression tag
Val 33	-	expression tag
Ala 34	-	expression tag
Ser 35	-	expression tag
Val 36	-	expression tag
Leu 37	-	expression tag
Ala 38	-	expression tag
Asp 767	-	linker
Pro 768	-	linker
Leu 769	-	linker
Val 770	-	linker
Pro 771	-	linker
Arg 772	-	linker
Gly 773	-	linker
Ser 774	-	linker
Gly 775	-	linker
Gly 776	-	linker
Gly 777	-	linker
Gly 778	-	linker
Asp 779	-	linker
Pro 780	-	linker

A, C: 781 - 1012 (UniProt: P01857)
B: 371 - 610 (UniProt: A0A2R4KP93)

PDB	External Database	Details
Met 355	-	initiating methionine
Arg 356	-	expression tag
Leu 357	-	expression tag
Ser 358	-	expression tag
Val 359	-	expression tag
Cys 360	-	expression tag
Leu 361	-	expression tag
Leu 362	-	expression tag
Met 363	-	expression tag
Phe 364	-	expression tag
Leu 365	-	expression tag
Leu 366	-	expression tag
Thr 367	-	expression tag
Pro 368	-	expression tag
Ile 369	-	expression tag
Lys 370	-	expression tag
Asp 611	-	linker
Pro 612	-	linker
Leu 613	-	linker
Val 614	-	linker
Pro 615	-	linker
Arg 616	-	linker
Gly 617	-	linker
Ser 618	-	linker
Gly 619	-	linker
Gly 620	-	linker
Gly 621	-	linker
Gly 622	-	linker
Asp 623	-	linker
Pro 624	-	linker

B: 625 - 856 (UniProt: P01857)

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	284158.2
Branched	Number of molecules	8
	Total formula weight	3557.3
Non-Polymers*	Number of molecules	8
	Total formula weight	1769.7
All*	Total formula weight	289485.2

*Water molecules are not included.