9JMH

Cryo-EM structure of HKU25-BatCoV S-trimer stabilized with 2P and x1 disulfide bond

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C (A, B, C)	Spike glycoprotein,Fibritin	polymer	1368	151971.3	3	UniProt (A0A2I4R888) UniProt (P10104)	Hypsugo bat coronavirus HKU25	S glycoprotein,E2,Peplomer protein,Collar protein,Whisker antigen control protein
2	D, F, H, I, J... (D, F, H, I, J...)	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		424.4	10	In PDB GlyTouCan (G42666HT)
3	E, G, K, L, P... (E, G, K, L, P...)	beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		586.5	6	In PDB GlyTouCan (G15407YE)
4	HA, T, UA (A, B)	LINOLEIC ACID	non-polymer		280.4	3	Chemie (EIC)
5	AA, AB, BA, BB, CA... (A, C, B)	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	38	Chemie (NAG)

Sequence modifications

A, B, C: 1 - 1289 (UniProt: A0A2I4R888)

PDB	External Database	Details
Cys 437	Ser 437	engineered mutation
Cys 1051	Asp 1051	engineered mutation
Pro 1052	Ala 1052	engineered mutation
Pro 1053	Val 1053	engineered mutation
Gly 1290	-	linker
Ser 1291	-	linker

A, B, C: 1292 - 1318 (UniProt: P10104)

PDB	External Database	Details
Leu 1313	Phe 479	conflict
Leu 1319	-	expression tag
Glu 1320	-	expression tag
Val 1321	-	expression tag
Leu 1322	-	expression tag
Phe 1323	-	expression tag
Gln 1324	-	expression tag
Gly 1325	-	expression tag
Pro 1326	-	expression tag
Gly 1327	-	expression tag
His 1328	-	expression tag
His 1329	-	expression tag
His 1330	-	expression tag
His 1331	-	expression tag
His 1332	-	expression tag
His 1333	-	expression tag
His 1334	-	expression tag
His 1335	-	expression tag
Ser 1336	-	expression tag
Ala 1337	-	expression tag
Trp 1338	-	expression tag
Ser 1339	-	expression tag
His 1340	-	expression tag
Pro 1341	-	expression tag
Gln 1342	-	expression tag
Phe 1343	-	expression tag
Glu 1344	-	expression tag
Lys 1345	-	expression tag
Gly 1346	-	expression tag
Gly 1347	-	expression tag
Gly 1348	-	expression tag
Ser 1349	-	expression tag
Gly 1350	-	expression tag
Gly 1351	-	expression tag
Gly 1352	-	expression tag
Gly 1353	-	expression tag
Ser 1354	-	expression tag
Gly 1355	-	expression tag
Gly 1356	-	expression tag
Ser 1357	-	expression tag
Ala 1358	-	expression tag
Trp 1359	-	expression tag
Ser 1360	-	expression tag
His 1361	-	expression tag
Pro 1362	-	expression tag
Gln 1363	-	expression tag
Phe 1364	-	expression tag
Glu 1365	-	expression tag
Lys 1366	-	expression tag
Ser 1367	-	expression tag
Ala 1368	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	455913.8
Branched	Number of molecules	16
	Total formula weight	7763.3
Non-Polymers*	Number of molecules	41
	Total formula weight	9247.2
All*	Total formula weight	472924.3

*Water molecules are not included.