9JMH
Cryo-EM structure of HKU25-BatCoV S-trimer stabilized with 2P and x1 disulfide bond
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B, C (A, B, C) | Spike glycoprotein,Fibritin | polymer | 1368 | 151971.3 | 3 | UniProt (A0A2I4R888) UniProt (P10104) Pfam (PF16451) Pfam (PF09408) Pfam (PF19209) Pfam (PF01601) | Hypsugo bat coronavirus HKU25 | S glycoprotein,E2,Peplomer protein,Collar protein,Whisker antigen control protein |
| 2 | D, F, H, I, J... (D, F, H, I, J...) | 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 424.4 | 10 | In PDB GlyTouCan (G42666HT) | |||
| 3 | E, G, K, L, P... (E, G, K, L, P...) | beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 586.5 | 6 | In PDB GlyTouCan (G15407YE) | |||
| 4 | HA, T, UA (A, B) | LINOLEIC ACID | non-polymer | 280.4 | 3 | Chemie (EIC) | |||
| 5 | AA, AB, BA, BB, CA... (A, C, B) | 2-acetamido-2-deoxy-beta-D-glucopyranose | non-polymer | 221.2 | 38 | Chemie (NAG) |
Sequence modifications
A, B, C: 1 - 1289 (UniProt: A0A2I4R888)
A, B, C: 1292 - 1318 (UniProt: P10104)
| PDB | External Database | Details |
|---|---|---|
| Cys 437 | Ser 437 | engineered mutation |
| Cys 1051 | Asp 1051 | engineered mutation |
| Pro 1052 | Ala 1052 | engineered mutation |
| Pro 1053 | Val 1053 | engineered mutation |
| Gly 1290 | - | linker |
| Ser 1291 | - | linker |
| PDB | External Database | Details |
|---|---|---|
| Leu 1313 | Phe 479 | conflict |
| Leu 1319 | - | expression tag |
| Glu 1320 | - | expression tag |
| Val 1321 | - | expression tag |
| Leu 1322 | - | expression tag |
| Phe 1323 | - | expression tag |
| Gln 1324 | - | expression tag |
| Gly 1325 | - | expression tag |
| Pro 1326 | - | expression tag |
| Gly 1327 | - | expression tag |
| His 1328 | - | expression tag |
| His 1329 | - | expression tag |
| His 1330 | - | expression tag |
| His 1331 | - | expression tag |
| His 1332 | - | expression tag |
| His 1333 | - | expression tag |
| His 1334 | - | expression tag |
| His 1335 | - | expression tag |
| Ser 1336 | - | expression tag |
| Ala 1337 | - | expression tag |
| Trp 1338 | - | expression tag |
| Ser 1339 | - | expression tag |
| His 1340 | - | expression tag |
| Pro 1341 | - | expression tag |
| Gln 1342 | - | expression tag |
| Phe 1343 | - | expression tag |
| Glu 1344 | - | expression tag |
| Lys 1345 | - | expression tag |
| Gly 1346 | - | expression tag |
| Gly 1347 | - | expression tag |
| Gly 1348 | - | expression tag |
| Ser 1349 | - | expression tag |
| Gly 1350 | - | expression tag |
| Gly 1351 | - | expression tag |
| Gly 1352 | - | expression tag |
| Gly 1353 | - | expression tag |
| Ser 1354 | - | expression tag |
| Gly 1355 | - | expression tag |
| Gly 1356 | - | expression tag |
| Ser 1357 | - | expression tag |
| Ala 1358 | - | expression tag |
| Trp 1359 | - | expression tag |
| Ser 1360 | - | expression tag |
| His 1361 | - | expression tag |
| Pro 1362 | - | expression tag |
| Gln 1363 | - | expression tag |
| Phe 1364 | - | expression tag |
| Glu 1365 | - | expression tag |
| Lys 1366 | - | expression tag |
| Ser 1367 | - | expression tag |
| Ala 1368 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 3 |
| Total formula weight | 455913.8 | |
| Branched | Number of molecules | 16 |
| Total formula weight | 7763.3 | |
| Non-Polymers* | Number of molecules | 41 |
| Total formula weight | 9247.2 | |
| All* | Total formula weight | 472924.3 |
