9JLX
Crystal structure of GH57 family amylopullulanase from Aquifex aeolicus mutant D352N in complex with maltoheptaose
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B, C, D (A, B, C, D) | Glycoside hydrolase family 57 N-terminal domain-containing protein | polymer | 477 | 57252.3 | 4 | UniProt (O66934) Pfam (PF03065) | Aquifex aeolicus VF5 | |
| 2 | E (E) | alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose | branched | 990.9 | 1 | In PDB GlyTouCan (G91368RN) | |||
| 3 | F, G, H (F, G, H) | alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose | branched | 1153.0 | 3 | In PDB GlyTouCan (G41288IQ) | |||
| 4 | I (A) | alpha-D-glucopyranose | non-polymer | 180.2 | 1 | Chemie (GLC) | |||
| 5 | J, K (A, D) | GLYCEROL | non-polymer | 92.1 | 2 | Chemie (GOL) PubChem (753) | |||
| 6 | L, M, N, O (A, B, C, D) | water | water | 18.0 | 1294 | Chemie (HOH) |
Sequence modifications
A, B, C, D: 1 - 477 (UniProt: O66934)
| PDB | External Database | Details |
|---|---|---|
| Asn 352 | Asp 352 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 4 |
| Total formula weight | 229009.0 | |
| Branched | Number of molecules | 4 |
| Total formula weight | 4449.9 | |
| Non-Polymers* | Number of molecules | 3 |
| Total formula weight | 364.3 | |
| All* | Total formula weight | 233823.2 |
