9JLV
Crystal structure of GH57 family amylopullulanase from Aquifex aeolicus mutant E256Q in complex with alpha-cyclodextrin
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Glycoside hydrolase family 57 N-terminal domain-containing protein | polymer | 477 | 57252.3 | 1 | UniProt (O66934) Pfam (PF03065) | Aquifex aeolicus VF5 | |
| 2 | B (B) | alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose | branched | 666.6 | 1 | In PDB GlyTouCan (G87171PZ) | |||
| 3 | C (C) | Cyclohexakis-(1-4)-(alpha-D-glucopyranose) | branched | 990.9 | 1 | In PDB GlyTouCan (G22307HL) | |||
| 4 | D, E (A) | CITRATE ANION | non-polymer | 189.1 | 2 | Chemie (FLC) | |||
| 5 | F, G (A) | GLYCEROL | non-polymer | 92.1 | 2 | Chemie (GOL) | |||
| 6 | H (A) | water | water | 18.0 | 340 | Chemie (HOH) |
Sequence modifications
A: 1 - 477 (UniProt: O66934)
| PDB | External Database | Details |
|---|---|---|
| Gln 256 | Glu 256 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 1 |
| Total formula weight | 57252.3 | |
| Branched | Number of molecules | 2 |
| Total formula weight | 1657.4 | |
| Non-Polymers* | Number of molecules | 4 |
| Total formula weight | 562.4 | |
| All* | Total formula weight | 59472.1 |
